Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Discrete wavelet transform-partial least squares versus derivative ratio spectrophotometry for simultaneous determination of chlorpheniramine maleate and dexamethasone in the presence of parabens in pharmaceutical dosage form

Hany W Darwish^1,2 , Fadia H Metwally^2,3, Abdelaziz El Bayoumi²

¹Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia; ²Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, ET 11562, Cairo, Egypt, 3Ibn Sina National College for Medical Studies, AlMahjer Road, Jeddah, Saudi Arabia.

For correspondence:- Hany Darwish Email: hdarwish75@yahoo.com Tel:+96614677343

Received: 5 January 2015 Accepted: 27 April 2015 Published: 26 May 2015

Citation: Darwish HW, Metwally FH, Bayoumi AE. Discrete wavelet transform-partial least squares versus derivative ratio spectrophotometry for simultaneous determination of chlorpheniramine maleate and dexamethasone in the presence of parabens in pharmaceutical dosage form. Trop J Pharm Res 2015; 14(5):859-867 doi: 10.4314/tjpr.v14i5.17

© 2015 The authors.
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Abstract

Purpose: To compare two methods, based on different approaches, for simultaneous determination of chlorpheniramine maleate (CHP) and dexamethasone (DX) in the presence of methyl and propyl paraben in phenadone syrup.

Methods: The first method used, based on univariate calibration approach, was first derivative of the ratio spectrophotometry (DD1). The second method, which is a multivariate calibration approach, was discrete wavelet transform followed by partial least squares method (DWT-PLS) which anticipated high predictive ability for the determination of both CHP and DX.

Results: DD1 method failed to determine DX due to the absence of adequate zero crossing point while DWT-PLS method was successfully applied for the analysis of raw materials and the dosage form. For DD1 method, recovery of chlorpheniramine maleate in the dosage form was 100.33 ± 0.91 % while for DWT-PLS method, recovery of chlorpheniramine maleate and dexamethasone was 100.24 ± 1.21 and 99.99 ± 1.08 %, respectively. The proposed methods were validated using standard addition technique and the results compared favorably with those obtained by a reference high performance liquid chromatography (HPLC) method.

Conclusion: The findings of this work show the superiority of DWT-PLS over DD1 method in solving such complex mixtures, and would thus be suitable for use in quality control (QC) laboratories and pharmaceutical industry.

Keywords: Quantitative analysis, Discrete wavelet transform, First derivative of ratio spectra, Chlorpheniramine maleate, Dexamethasone