Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vivo Evaluation of Amoxicillin Trihydrate and Clarithromycin-Loaded Mucoadhesive Microspheres for H. pylori Eradication

Venkateswaramurthy Nallasamy¹ , Sambathkumar Ramanathan¹, Perumal Perumal²

¹Department of Pharmaceutics; ²Department of Pharmaceutical Chemistry, J.K.K. Nataraja College of Pharmacy, Komarapalayam, Namakkal District, Tamilnadu. India.

For correspondence:- Venkateswaramurthy Nallasamy Email: nvmurthi@gmail.com Tel:+914256234888

Received: 17 July 2012 Accepted: 21 February 2013 Published: 24 April 2013

Citation: Nallasamy V, Ramanathan S, Perumal P. In vivo Evaluation of Amoxicillin Trihydrate and Clarithromycin-Loaded Mucoadhesive Microspheres for H. pylori Eradication. Trop J Pharm Res 2013; 12(2):143-147 doi: 10.4314/tjpr.v12i2.2

© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate in vivo H. pylori clearance efficacy of formulated mucoadhesive microspheres of amoxicillin trihydrate and clarithromycin.

Methods: Amoxicillin trihydrate and clarithromycin mucoadhesive microspheres were prepared by solvent evaporation method using Carbopol 974P, HPMC K4M and Eudragit RS 100. In vivo clearance efficacy of the microspheres was evaluated in a Wistar rat model after induction of H. pylori infection. Amoxicillin and clarithromycin-loaded microspheres were administered twice daily for three days. H. Pylori clearance was evaluated by assessing colony count.

Results: Treatment with plain drug solution (90 mg/kg amoxicillin and 45 mg/kg clarithromycin) resulted in a colony count of log 1.25 ± 0.56 CFU and clearance rate of 60 %, while mucoadhesive microspheres-loaded dose of 45 mg/kg amoxicillin and 22.5 mg/kg clarithromycin resulted in complete (100 %) eradication of H. pylori infection.

Conclusion: The developed mucoadhesive amoxicillin/clarithromycin microspheres can potentially be used to effectively eradicate H. pylori infection.

Keywords: Amoxicillin, Carbopol, Clarithromycin, H. pylori, Hydroxypropyl methylcellulose, Microspheres, Mucoadhesive