Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Development and Validation of Analytical Method for Losartan-Copper Complex Using UV-Vis Spectrophotometry

Syed S ul Hassan¹, Syeda FA Zaidi¹, Imran Tariq¹ , Muhammad T Ansari²

¹University College of Pharmacy, University of the Punjab, Lahore-54000; ²Department of Pharmacy, Bahaudin Zakariya University, Multan, Pakistan.

For correspondence:- Imran Tariq Email: imran1982@hotmail.com Tel:00923224218583

Received: 3 August 2012 Accepted: 26 April 2013 Published: 12 June 2013

Citation: Hassan SS, Zaidi SF, Tariq I, Ansari MT. Development and Validation of Analytical Method for Losartan-Copper Complex Using UV-Vis Spectrophotometry. Trop J Pharm Res 2013; 12(3):407-411 doi: 10.4314/tjpr.v12i3.20

© 2013 The authors.
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Abstract

Purpose: To develop a new spectrophotometric method for the analysis of losartan potassium in pharmaceutical formulations by making its complex with copper.

Method: A coloured complex based on UV/Vis spectroscopic method was developed for the determination of losartan potassium concentration in pharmaceutical formulations in the visible region. The colored complex of losartan was formed with cupric acetate (5:4). Analysis was carried out by the two methods - absorption ratio and calibration curve methods. The proposed method was validated in terms of linearity, accuracy and precision.

Results: The λ maximum of the complex was recorded at 530 nm. Beer`s law was obeyed in the range of 10 - 50 µg/ml with a correlation coefficient (r²) of 0.9989. Mean analytical results from the calibration curve and absorption ratio methods were 99.42 and 99.16 % with relative standard deviation (RSD) of 0.97 and 0.82 %, respectively. Mean recovery was between 98.61 and 101.35 % and precision expressed as relative standard deviation (RSD) was 0.91 %.

Conclusion: The proposed method is simple, easy to apply, low-cost, and requires relatively inexpensive instruments. Thus, it is a suitable alternative to currently used spectrophotometric methods for the determination of losartan in bulk and solid dosage forms.

Keywords: Losartan, Copper complex, Spectrophotometry, Validation