Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Simultaneous Determination of Co-formulated Matrine and Secnidazole in Suppositories by Reverse Phase High Performance Liquid Chromatography

Haixia Lv, Suying Ma , Xiao Wang, Xiaojun Shang

School of Pharmacy, Xinxiang Medical University, Xinxiang Henan, 453003, PR China;

For correspondence:- Suying Ma Email: suyingma@163.com Tel:+863733029879

Received: 20 February 2012 Accepted: 28 March 2013 Published: 12 June 2013

Citation: Lv H, Ma S, Wang X, Shang X. Simultaneous Determination of Co-formulated Matrine and Secnidazole in Suppositories by Reverse Phase High Performance Liquid Chromatography. Trop J Pharm Res 2013; 12(3):413-418 doi: 10.4314/tjpr.v12i3.21

© 2013 The authors.
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Abstract

Purpose: To develop and validate a new, simple, sensitive and accurate high performance liquid chromatographic (HPLC) method for the simulataneous determination of matrine and secnidazole in suppositories.

Methods: The method involved using a SinoChoom ODS-BP C₁₈ column (5 μm, 4.6 mm × 200 mm) and mobile phase consisting of acetonitrile–triethylamine (0.05 %) in 0.025 mol/L KH₂PO₄ (20:80, v/v). The flow rate was 1 mL/min and detection was monitored at 210 and 311 nm for matrine and secnidazole, respectively. Total run time was 10 min and the column was maintained at 35 ^oC.

Results: The excipients in the suppository did not interfere with the drug peaks. Matrine was eluted at a retention time (RT) of 4.30 min while linearity for the quantification of drug was obtained in the concentration range of 10.0 - 100.0 µg/mL (r² = 0.9991). Secnidazole was eluted at a retention time (tr) of 6.69 min and linearity for the quantification of the drug was obtained in the concentration range of 10.0 - 150.0 µg/mL (r² = 0.9993). Intra- and inter-day variations were < 1.0 % for both matrine and secnidazole.

Conclusion: The developed HPLC method was validated according to International Conference on Harmonisation (ICH) guidelines and proved to be suitable for the simultaneous determination of matrine and secnidazole in suppositories.

Keywords: Matrine, Secnidazole, Suppository, HPLC, Assay