Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Anionic Polymers on Drug Loading and Release from Clindamycin Phosphate Solid Lipid Nanoparticles

Mohammadreza Abbaspour¹ , Behzad Sharif Makhmalzadeh^1,2, Zahra Arastoo¹, Alireza Jahangiri^1,2, Roohollah Shiralipour^2,3

¹Nanotechnology Research Center and School of Pharmacy; ²Food and Drug Safety Evaluation Research Center, Ahvaz Jundishapur University of Medical Sciences; ³Department of Chemistry, Shahid Chamran University, Ahvaz, Iran.

For correspondence:- Mohammadreza Abbaspour Email: abbaspourmr@ajums.ac.ir Tel:+00986113738380

Received: 6 July 2012 Accepted: 22 June 2013 Published: 23 August 2013

Citation: Abbaspour M, Makhmalzadeh BS, Arastoo Z, Jahangiri A, Shiralipour R. Effect of Anionic Polymers on Drug Loading and Release from Clindamycin Phosphate Solid Lipid Nanoparticles. Trop J Pharm Res 2013; 12(4):477-482 doi: 10.4314/tjpr.v12i4.5

© 2013 The authors.
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Abstract

Purpose: To develop and characterize solid lipid nanoparticle (SLN) systems containing dextran sulfate or sodium alginate as anionic polymers for the delivery of clindamycin phosphate as a model hydrophilic cationic drug.

Methods: A multi-level factorial design was used for the preparation and optimization of clindamycin SLNs. Polymers (dextran sulfate and sodium alginate), Tween 80, and Pluronic F68 were chosen as the independent variables. The SLNs were prepared using stearic acid as the lipid matrix by an emulsion congealing technique with cold homogenization. Particle size and drug loading were evaluated as the primary responses. The morphology and drug release rate of the selected formulations were also determined.

Results: The results revealed that incorporation of anionic polymers increased drug loading of the SLNs. Dextran sulfate had a greater effect on drug loading, increasing it from 1.32 to 18.19 %, compared to the 6.73 % achieved using sodium alginate. Dextran sulfate also reduced drug release rate by half compared with sodium alginate, probably due to the higher charge density, lower molecular weight and lower branching density of the ionic polymer.

Conclusion: Incorporation of anionic polymers can increase the loading of clindamycin phosphate into SLNs. Drug release from SLNs is also dependent on the polymer type.

Keywords: Clindamycin, Solid lipid nanoparticles, Dextran sulfate, Sodium alginate, Anionic polymers, Drug release, Drug loading