Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Inhibitory Effect of Polysaccharides from Scutellaria barbata D. Don on Invasion and Metastasis of 95-D Cells Lines via Regulation of C-MET and E-CAD expressions

Xiao-Kun Yang^1,2, Ya-Dong Yang¹, Shu-Qian Tang¹, Ling Xu¹, Gui-Hong Yang¹, Qiao-Yu Xu¹, Hui Tang¹, Jin-Jin Wu¹

¹Department of Dermatology, Research Institute of Wound Healing and Tissue Engineering of Chongqing, Daping Hospital, The Third Military Medical University, Chongqing, 400042; ²Department of Emergency, General Hospital of Chengdu Military Command Area, Chengdu 610083, Sichuan Province, PR China.

For correspondence:- Jin-Jin Wu Email: wujjtmmu@126.com Tel:+8602368757599

Received: 17 April 2013 Accepted: 3July 2013 Published: 23 August 2013

Citation: Yang X, Yang Y, Tang S, Xu L, Yang G, Xu Q, et al. Inhibitory Effect of Polysaccharides from Scutellaria barbata D. Don on Invasion and Metastasis of 95-D Cells Lines via Regulation of C-MET and E-CAD expressions. Trop J Pharm Res 2013; 12(4):517-522 doi: 10.4314/tjpr.v12i4.11

© 2013 The authors.
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Abstract

Purpose: To investigate the inhibitory effect of polysaccharides from Scutellaria barbata (PSB) on invasion and metastasis of lung cancer, and study the possible mechanism.

Methods: PSB was extracted with water and by alcohol precipitation, and purified by DEAE-52 column chromatography. A highly invasive and metastatic lung carcinoma cell, 95-D cell line, was used for the study. Cell adhesion and invasion assays in vitro were performed to evaluate the anti-invasive and anti-metastatic effects of PSB (50 - 200 μg/ml) on 95-D cell. Immunocytochemical staining and Western blot techniques were employed to study the regulatory effects of PSB on the expression of C-MET and E-CAD.

Results: The results indicate that PSB significantly inhibited cell invasion and migration of 95-D in a concentration-dependent manner (p < 0.05). The adhesion of 95-D cells to fibronectin was also inhibited by PSB (p < 0.05). The expression of C-MET and E-CAD in 95-D cells treated with PSB were significantly down-regulated and up-regulated, respectivelt (p < 0.05).

Conclusion: Treatment with PSB can significantly inhibit the invasion and metastasis of 95-D cells in vitro, probably through the regulation of C-MET and E-CAD.

Keywords: Polysaccharide, Scutellaria barbata, 95-D cell lines, Invasion, Metastasis