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Original Research Article | OPEN ACCESS

Quercetin - A Flavonoid Compound from Sarcopyramis bodinieri var delicate with Potential Apoptotic Activity in HepG2 Liver Cancer Cells

Weifeng Huang2, Chunpeng Wan1 , Shouran Zhou3

1Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045; 2Medical College, China Three Gorges University, Yichang 443002; 3Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.

For correspondence:-  Chunpeng Wan   Email: lemonwan@126.com   Tel:+8679183813185

Received: 25 April 2013        Accepted: 25 July 2013        Published: 23 August 2013

Citation: Huang W, Wan C, Zhou S. Quercetin - A Flavonoid Compound from Sarcopyramis bodinieri var delicate with Potential Apoptotic Activity in HepG2 Liver Cancer Cells. Trop J Pharm Res 2013; 12(4):529-533 doi: 10.4314/tjpr.v12i4.13

© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To isolate and identify the cytotoxicity of the constituents of Sarcopyramis bodinieri var. delicate.
Methods: S. bodinieri var. delicate was extracted with hydrochloric acid-methanol and fractionated with ethyl acetate further. The chemical constituents of the ethyl acetate fraction were purified by a combination of D101 macroporous resin and Sephadex LH-20 column chromatography. The structure was characterized by 1H-Nuclear Magnetic Resonance (NMR) and electrospray ionization tandem mass spectrometry (ESI-MS). Apoptosis was evaluated by fluorescence staining and Western blot analysis using 4,6-diamidino-2-phenylindole (DAPI) staining and poly (ADP-ribose) polymerase (PARP) SDS-PAGE tests in HepG2 liver cancer cells.
Results: One flavonoid with high purity was purified by the combination of D101 macroporous resin and Sephadex LH-20 column chromatography. The flavonoid compound was identified as quercetin by 1H-NMR and ESI-MS analyses. DAPI staining and PARP SDS-PAGE tests showed 60 mM quercetin could induce potential apoptotic activity in HepG2 liver cancer cells.
Conclusion: Quercetin was the major cytotoxicity constituent in S. bodinieri var. delicate.

Keywords: Apoptotic activity, Quercetin, Sarcopyramis bodinieri var. delicate, HepG2 liver cancer cells, Electrospray ionization tandem

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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