Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Acylated Flavonoid from Vaccinium Corymbosum (Ericaceae) Flowers with Yeast α-Glucosidase Inhibitory Activity

Chunpeng Wan¹ , Shouran Zhou²

¹Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045; ²Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.

For correspondence:- Chunpeng Wan Email: lemonwan@126.com Tel:+867913813185

Received: 21 September 2012 Accepted: 5 June 2013 Published: 23 August 2013

Citation: Wan C, Zhou S. Acylated Flavonoid from Vaccinium Corymbosum (Ericaceae) Flowers with Yeast α-Glucosidase Inhibitory Activity. Trop J Pharm Res 2013; 12(4):549-552 doi: 10.4314/tjpr.v12i4.16

© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To isolate and identify chemical constituents with yeast α-glucosidase inhibitory activity from the methanol extract of V. myrtillus (blueberry) flowers.

Methods: The active fraction (ethyl acetate extract) were chromatographed on C18 MPLC column, Sephadex LH-20 column and semi-preparative HPLC column. The isolated compounds were identified by the extensive ¹H-nuclear magnetic resonance spectroscopy (NMR), ¹³C-NMR, 2D-NMR and high resolution mass spectral (HR-MS) analyses.

Results: Two phenolic compounds, an acylated flavonoid and a coumaric acid derivative, were isolated and identified as isorhamnetin-3-O-(6''-O-coumaroyl)-β-D-glucoside (1) and cis-cinnamic acid methyl (2). Compound 1 showed powerful α-glucosidase inhibitory activity and in this regard, was superior to the positive drug, acarbose.

Conclusion: Compounds 1 and 2 were isolated for the first time from this species and the genus of Vaccinium. This is the first report on characterization of these phenolic compounds and the possible utilization of blueberry flowers for nutraceutical and functional food applications.

Keywords: Vaccinium corymbosum, Blueberry, Acylated flavonoid, Yeast ^5;-Glucosidase, Inhibitory activity, Nutraceuticals