Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Enhancing the Production of a Novel Exopolysaccharide by Bacillus mucilaginosus CGMCC5766 Using Statistical Experiment Design

Hui Li¹, Jie Li¹, Wenfang Dou¹, Jinsong Shi¹ , Zhenghong Xu²

¹Laboratory of Pharmaceutical Engineering, School of Pharmaceutical Science, Jiangnan University; ²The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China.

For correspondence:- Jinsong Shi Email: shijs@163.com Tel:+8651085328177

Received: 18 October 2012 Accepted: 25 July 2013 Published: 18 October 2013

Citation: Li H, Li J, Dou W, Shi J, Xu Z. Enhancing the Production of a Novel Exopolysaccharide by Bacillus mucilaginosus CGMCC5766 Using Statistical Experiment Design. Trop J Pharm Res 2013; 12(5):711-718 doi: 10.4314/tjpr.v12i5.8

© 2013 The authors.
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Abstract

Purpose: To improve the production of a novel exopolysaccharide (EPS) by Bacillus mucilaginosus CGMCC5766.

Methods: The culture medium for production of EPS was optimized using statistical experiment design. Sucrose, CaCO₃and K₂HPO₄ were found to be the key factors based on the results obtained from Plackett–Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and then the optimum concentration was further investigated through response surface methodology. Validation experiments were also carried out to establish the adequacy and accuracy of the model obtained.

Results: Under optimal conditions (sucrose 61.31 g/l, CaCO₃5.06 g/l and K₂HPO₄ 0.17 g/l), EPS concentration achieved 22.04 g/l, which was about 1.6-fold increase compared with that using the original medium. EPS concentration in 5-l fermenter reached 23.46 ± 0.76 g/l.

Conclusion: The optimization medium is considered fundamental and useful for the development of B. mucilaginosus CGMCC5766 cultivation process for efficient production of EPS on a large scale.

Keywords: Bacillus mucilaginosus CGMCC5766, Exopolysaccharide, Medium, Optimization, Statistical experiment design