Blaga C Radovanović1,
Ana S Milenković Anđelković1 
,
Aleksandra B Radovanović1,
Marko Z Anđelković2
For correspondence:- Ana Anđelković Email: ana.andjelk@gmail.com Tel:+38116 218415
Received: 17 October 2012 Accepted: 8 July 2013 Published: 18 October 2013
Citation: Radovanović BC, Anđelković AS, Radovanović AB, Anđelković MZ. Antioxidant and Antimicrobial Activity of Polyphenol Extracts from Wild Berry Fruits Grown in Southeast Serbia. Trop J Pharm Res 2013; 12(5):813-819 doi: 10.4314/tjpr.v12i5.23
© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To assess the antioxidant and antimicrobial activities of polyphenolic extracts of three wild red wild berry fruit species from Southeast Serbia, viz, European cornel (Cornus mas), blackthorn (Prunus spinosa L.) and wild blackberry (Rubus fruticosus).
Methods: Polyphenol content was determined using spectrophotometric and High performance liquid chromatography (HPLC) methods. Antioxidant activity was estimated by 2,2`- diphenyl - 1 - picrylhydrazyl (DPPH) test while reliminary antimicrobial tests were carried out by disc diffusion method in which antibacterial activity was evaluated by measuring the zone of inhibition against test bacterial strains. Broth microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
Results: The highest amount of total phenols was found in European cornel (8625.89 mg kg-1FW). Galic acid, caffeic acid, p-coumaric acid, ferulic acid, (+)-catechin, procyanidin B2, (-)-epicatechin, quercetin, rutin and quercetin-3-glucoside were the main polyphenols in the fruit extracts. All extracts showed high scavenging effect on DPPH radical with IC50 values ranging from 22.19 to 31.18 ml g-1, as well as high antimicrobial activity on almost all the tested bacterial strains.
Conclusion: Extracts of wild berry fruits may be suitable for the preparation of medicinal and nutritional products.
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