Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Spectrophotometric Determination of Trimipramine in Tablet Dosage Form via Charge Transfer Complex Formation

Abdullah Al-Badr, Gamal Abdel-Hafiz Mostafa

Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia;

For correspondence:- Gamal Mostafa Email: gamalmost@yahoo.com

Received: 20 February 2013 Accepted: 23 October 2013 Published: 24 December 2013

Citation: Al-Badr A, Mostafa GA. Spectrophotometric Determination of Trimipramine in Tablet Dosage Form via Charge Transfer Complex Formation. Trop J Pharm Res 2013; 12(6):1057-1063 doi: 10.4314/tjpr.v12i6.29

© 2013 The authors.
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Abstract

Purpose: To develop and validate simple, rapid and sensitive spectrophotometric procedures for determination of trimipramine in tablet dosage form.

Methods: The methods were based on the interaction of trimipramine as n-electron donor with the s-acceptor, iodine and various p-acceptors, namely: chloranil (CH), chloranilic acid (ChA), 2,3-dichloro-5, 6-dicyano-1, 4-benzoquinone (DDQ), and 7, 7,8, 8 tetracyanoquinodimethane (TCNQ), to form charge transfer complexes. The complexes obtained were measured spectrophotometrically at 292, 220, 520, 302, and 824 nm for I₂, CH, ChA, DDQ, and TCNQ, respectively. Different variables affecting the reaction were carefully studied and optimized.

Results: Beer's law was obeyed over the concentration ranges 1 - 5, 5 - 50, 15 - 100, 5- 50, and 10 -75 ppm for I₂, CH, ChA, DDQ, and TCNQ respectively, with apparent molar absorptivities of 7.1 ´ 10⁴, 0.3 ´ 10⁴, 1.6 ´ 10⁴, 0.26 ´ 10⁴, and 0.1 ´ 10⁴ l mol^-1cm^-1respectively. The proposed methods were successfully applied to the determination of trimipramine with good accuracy and precision.

Conclusion: The results demonstrated that the developed methods are as accurate, precise and reproducible as the pharmacopeial method. The methods would be valuable for routine application in quality control.

Keywords: Charge-transfer complex, Trimipramine, Spectrophotometry (T and S)