Zheng-Quan Lai1,2,
Hui-Jun Liao1,2,
Siu-Po Ip2,
Yu-Yang Yi1,
Shu-Jiang Shi1,
Ji-Yan Su1,3,
Xiao-Ping Lai1,3,
Zi-Ren Su1 
,
Zhi-Xiu Lin2
For correspondence:- Zi-Ren Su Email: suziren@gzucm.edu.cn Tel:+862039358517
Received: 08 October 2013 Accepted: 20 November 2013 Published: 25 January 2014
Citation: Lai Z, Liao H, Ip S, Yi Y, Shi S, Su J, et al. Liquid Chromatography - Mass Spectrometry Method for the Simultaneous Determination and Confirmation of Seven Active Components in Chinese Medicine Kumu Injection. Trop J Pharm Res 2014; 13(1):141-148 doi: 10.4314/tjpr.v13i1.20
© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To develop and validate a simple and selective high performance liquid chromatography photo diode array mass spectrometry (HPLC-PDA-MS/MS) method for simultaneous determination and confirmation of seven major active alkaloids (6-Hydroxy-β-Carboline-1-carboxylic acid, β-Carboline-1-carboxylic acid, β-Carboline-1-propanoic acid, 3-Methylcanthin-5,6-dione, 4-Methoxy-3-methylcanthine-5,6-dione, 5-Hydroxy-4-methoxycanthin-6-one, 4,5-Dimethoxycanthin-6-one) in Kumu injections (KMIs).
Methods: For the analysis of the preparation, the optimal chromatographic condition was achieved on a Phenomenex Gemini C18 column with gradient elution of 25 mM aqueous ammonium acetate (pH = 4.0 adjusted by glacial acetate acid) and acetonitrile with @258;ow rate at 1.0 mL/min, column temperature at 35 oC and detection wavelengths at 245, 260 and 271 nm.
Results: Excellent linear behavior over the investigated concentration ranges was observed with regression coeffcient (R2) > 0.9997 for all analytes. Intra- and inter-day precisions for all studied constituents ranged from 0.20 to 1.80 %. Recoveries of the assayed constituents were in the range of 98.73 to 100.34 %. The results showed the contents of these seven marker compounds differed significantly among different batches of KMIs both from the same and different manufacturers.
Conclusion: The validated method was reliable, accurate, repeatable and can be applied to routine quality assessment of these active components in KMIs.
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