Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Pectolinarigenin - A Flavonoid Compound from Cirsium Japonicum with Potential Anti-proliferation Activity in MCF-7 Breast Cancer Cell

Mingqian Lu^1,2,3, Qingzhi Kong^1,4 , Xinhua Xu^2,3, Hongda Lu⁴, Zhongxin Lu⁴, Wei Yu^2,3, Bin Zuo^2,3, Jin Su^2,3, Rong Guo^2,3

¹Hubei University of Chinese Medicine, Wuhan 430061; ²Institute of Oncology, China Three Gorges University; ³Oncology Department, Yichang Central Hospital,Yichang 443000; ⁴Wuhan Central Hospital & Wuhan Institute of Oncology, Wuhan 430061, China.

For correspondence:- Qingzhi Kong Email: lumqyc@sina.cn Tel:+8607176486745

Received: 17 January 2013 Accepted: 6 January 2014 Published: 20 February 2014

Citation: Lu M, Kong Q, Xu X, Lu H, Lu Z, Yu W, et al. Pectolinarigenin - A Flavonoid Compound from Cirsium Japonicum with Potential Anti-proliferation Activity in MCF-7 Breast Cancer Cell. Trop J Pharm Res 2014; 13(2):225-228 doi: 10.4314/tjpr.v13i2.9

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To isolate high purity pectolinarigenin from Cirsium japonicum and evaluates its anti-proliferative potential and induction of apoptosis activity in MCF-7 breast cancer cell.

Methods: Pectolinarigenin was purified by the combination of silica gel and Sephadex LH-20 column chromatography. The structure was identified by ¹H-nuclear magnetic resonance (NMR), ¹³C-NMR and electrospray ionization tandem mass spectrometry (ESI-MS) analyses. The anti-proliferation activity of pectolinarigenin was also evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) and colony formation assays in MCF-7 breast cancer cell.

Results: Pectolinarigenin showed potent anti-proliferation activity by inducing apoptosis and downregulation of Bcl2 expression in MCF-7 breast cancer cell. Protein expression of Bcl2 was almost completely inhibited upon the treatment with 25 mM pectolinarigenin.

Conclusion: A rapid method to isolate high purity pectolinarigenin from C. japonicum and as well as evaluate its anti-proliferative potential and induction of apoptosis in MCF-7 breast cancer cells is presented for the first time. Induction of apoptosis of MCF-7 is via downregulation of Bcl2 expression.

Keywords: Cirsium japonicum, Anti-proliferation activity, Pectolinarigenin, Breast cancer, Apoptosis