Zu Qing Su1,
Xiao Li Wu1,
Mei Jia Bao2,
Chu Wen Li1,
Song Zhi Kong1,
Zi Ren Su1,3,
Xiao Ping Lai1,3,
Yu Cui Li1,
Jian Nan Chen1,3 
For correspondence:- Jian Chen Email: chenjiannan@gzucm.edu.cn Tel:+862039358517
Received: 13 August 2012 Accepted: 12 January 2014 Published: 24 March 2014
Citation: Su ZQ, Wu XL, Bao MJ, Li CW, Kong SZ, Su ZR, et al. Isolation of (-)-Patchouli Alcohol from Patchouli Oil by Fractional Distillation and Crystallization. Trop J Pharm Res 2014; 13(3):359-363 doi: 10.4314/tjpr.v13i3.7
© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To establish a new and efficient method for the isolation of (-)-patchouli alcohol (PA) from patchouli oil (PO).
Methods: PO, obtained from commercial source, was separated into four fractions (A, B, C and raffinate) using fractional distillation according to pre-set reflux ratio in vacuum. PA was crystallized from fraction C (containing more than 80 % PA by weight) by cooling and centrifugation. Finally, PA was further purified by suction filtration. Characterization of PA was performed by melting point (MP), infrared spectroscopy (IR), 1H and 13C nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS).
Results: The total yield of PA in this procedure reached 52.9 %. The structure of PA was obtained based on data from 1H-NMR, 13C-NMR and MS analysis with the aid of literature data for authenticated samples.
Conclusion: Fractional distillation combined with crystallization can be successfully applied to the isolation of PA from PO in solvent-free conditions.
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