Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Resuscitation Fluids on the expression of hsp90α in Cardiac Muscles of Hemorrhagic Shocked Rats

Xiao-Kun Yang¹, Jie Su², Gui-Sen Xu¹

¹Department of Emergency, General Hospital of Chengdu Military Command Area, Chengdu 610083; ²Department of Emergency, Chengdu First Peopleâ€™s Hospital, Chengdu 610016, Sichuan Province, PR China.

For correspondence:- Gui-Sen Xu Email: guisenxucdzy@126.com Tel:+8602886570325

Received: 20 October 2013 Accepted: 23 January 2014 Published: 24 March 2014

Citation: Yang X, Su J, Xu G. Effect of Resuscitation Fluids on the expression of hsp90α in Cardiac Muscles of Hemorrhagic Shocked Rats. Trop J Pharm Res 2014; 13(3):365-368 doi: 10.4314/tjpr.v13i3.8

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the protective effects of various resuscitating fluids on severe hemorrhagic shocked (HS) rats by comparing the expression changes of hsp90α in cardiac muscles and survival of rats.

Methods: Western-blot and immunohistochemistry methods were performed to determine hsp90α expressions in cardiac muscles of HS rats following treatment with different resuscitation fluids. In addition, the survival rates of of all the test groups were investigated.

Results: The expression of hsp90α decreased following treatment with resuscitation fluids based on both Western blot and immunohistochemistry data. Ringer lactate solution (RLS) was the most effective therapeutic fluid in this regard, and this was buttressed by survival rate data (90 %).

Conclusion: The expression of hsp90α in rat cardiac muscle is decreased to various degrees by treatment with different resuscitating fluids, with the effect most pronounced for ringer lactate solution. Furthermore, hsp90α plays an important role in hemorrhagic rat cardiac muscle as a regulatory factor.

Keywords: Resuscitating fluids, Hemorrhagic shock, Heat shock protein 90a, hsp90a