Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro Antiglycation and Cross-Link Breaking Activities of Sri Lankan Low-Grown Orthodox Orange Pekoe Grade Black Tea (Camellia sinensis L)

Wanigasekara D Ratnasooriya¹ , Walimuni KSM Abeysekera², Tharaka BS Muthunayake¹, Chatura DT Ratnasooriya¹

¹Department of Zoology, University of Colombo, Colombo 03; ²Food Technology Section, Industrial Technology Institute, Colombo 07, Sri Lanka.

For correspondence:- Wanigasekara Ratnasooriya Email: wdr@zoology.cmb.ac.lk Tel:+94112503399

Received: 1 February 2013 Accepted: 5 February 2014 Published: 23 April 2014

Citation: Ratnasooriya WD, Abeysekera WK, Muthunayake TB, Ratnasooriya CD. In vitro Antiglycation and Cross-Link Breaking Activities of Sri Lankan Low-Grown Orthodox Orange Pekoe Grade Black Tea (Camellia sinensis L). Trop J Pharm Res 2014; 13(4):567-571 doi: 10.4314/tjpr.v13i4.12

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the antiglycation and cross-link breaking activities of Sri Lankan low-grown orthodox Orange Pekoe grade black tea (Camellia sinensis L).

Methods: Five concentrations (6.25, 12.5, 25.0, 50.0 or 100.0 µg/ml) of Black tea brew (BTB) were made using Sri Lankan low-grown Orange Pekoe (O.P.) grade tea. Antiglycation and advanced glycation end products (AGEs) cross-link breaking activities of BTB as well as the antiglycation activity of rutin were determined in vitro on bovine serum albumin/glucose system using fluorescence spectroscopy.

Results: BTB induced significant (p<0.05) antiglycation activity (IC₅₀, 19.04 ± 5.18 µg/ml) and AGEs cross-link breaking activities (IC₅₀, 82.89 ± 3.44 µg/ml). These effects were dose-dependent. Further, the antiglycation activity of BTB was comparable to rutin, a well-known antiglycation agent (IC₅₀, 21.88 ± 2.82 µg/ml).

Conclusion: These results show that Sri Lankan low-grown O.P. grade black tea possesses both antiglycation and AGEs cross-link breaking activities in vitro and thus provides scientific justification for the use of black tea in Sri Lankan indigenous medicine for the management of diabetic complications.

Keywords: Diabetic complications, Antiglycation, Glycotoxin, Black tea, Cross-link breakers, Sri Lankan tea, Orange Pekoe tea