Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Optimization and Composition of Volatile Oil from Polygonatum odoratum (Mill Druce) using Supercritical Fluid Extraction

Xiuhong Zhao¹ , Jie Zeng², Haiyan Gao², Ying Wang³

¹Grain College, Shen Yang Normal University, Shenyang 110034; ²School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003; ³Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

For correspondence:- Xiuhong Zhao Email: zhaoxiuhong1976@aliyun.com Tel:+862486506009

Received: 1 March 2013 Accepted: 14 March 2014 Published: 23 May 2014

Citation: Zhao X, Zeng J, Gao H, Wang Y. Optimization and Composition of Volatile Oil from Polygonatum odoratum (Mill Druce) using Supercritical Fluid Extraction. Trop J Pharm Res 2014; 13(5):779-786 doi: 10.4314/tjpr.v13i5.19

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the effects of temperature, pressure and extraction time on oil yield obtained from Polygonatum odoratum as well as the optimum processing conditions for supercritical carbon dioxide (CO₂) extraction.

Methods: Supercritical CO₂ extraction technology was adopted in this experiment to study the process of extraction of volatile oil from Polygonatum odoratum while gas chromatograph-mass spectrometer (GC-MS) technique was employed to analyze the chemical composition of the volatile oil. Response surface methodology (RSM) was applied to optimize supercritical CO₂ process conditions.

Results: The determined optimal conditions were as follows: extraction pressure, 27 MPa; extraction temperature, 50 °C; and extraction time, 97.10 min. Under these conditions, the predicted yield of Polygonatum volatile oil was 2.04 % as against an actual oil yield of 2.02 % of the seeds. GC-MS analysis showed that the extract obtained by supercritical CO₂ extraction was richer in fatty acids and flavoring substances than that obtained by hydrodistillation.

Conclusion: Using RSM can optimize conditions of Polygonatum volatile oil. This provides a scientific basis for further research and development of healthy foods, as well as comprehensive utilization of Polygonatum odoratum.

Keywords: Polygonatum odoratum, Volatile oil, Supercritical fluid, Carbon dioxide, Extraction, Gas chromatograph-mass spectrometer