Neetu Sachan1 
,
Phool Chandra1,
Dilipkumar Pal2
For correspondence:- Neetu Sachan Email: neetuphool@gmail.com Tel:+915912360022
Received: 16 October 2014 Accepted: 26 April 2015 Published: 29 June 2015
Citation: Sachan N, Chandra P, Pal D. Assessment of gastroprotective potential of Delonix regia (Boj Ex Hook) Raf against ethanol and cold restrain stress-induced ulcer in rats. Trop J Pharm Res 2015; 14(6):1063-1070 doi: 10.4314/tjpr.v14i6.18
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To assess the gastroprotective potential of the stem bark ethanol extract of Delonix regia (EDR) on ethanol and cold restrain stress-induced ulcer in experimental rats.
Methods: EDR (100, 200 and 400 mg/kg doses, orally) was evaluated on ethanol and cold restrain stress-induced ulcer in experimental rats. In ethanol induced ulcer model, ulcer index, percent protection, reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO), cytokines and nitric oxide (NO) levels in stomach tissue were evaluated. In the cold restrain stress model, ulcer index, percent protection, and GSH levels were evaluated. 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging assay of EDR was also performed.
Results: EDR caused a significant (p < 0.05-0.001) decreased ulcer index in ethanol (61.33-76.00%) and cold restrain stress (47.34-84.28 %) models. The EDR caused a significant (p < 0.05 - 0.001) increase in SOD (0.20 - 0.27 U/mg protein, CAT (200 - 270 µmole H2O2/mg of protein/minute), GSH (1.63 - 1.17 µg/mg protein) and reduction in nitric oxide (NO) level, pro-inflammatory cytokine (TNF-α and IL-6) levels and inhibition in neutrophil accumulation (p < 0.001) in ethanol-induced model. EDR exhibited significant antioxidant activity with IC50 value of 45.23 ± 3.23 μg/ml.
Conclusion: The results suggest that EDR has gastroprotective effect in the two ulcer models and this may be due to its antioxidant effect.
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