Yu Jae Hyun1,
Mei Jing Piao1,
Ki Cheon Kim1,
Jian Zheng1,
Cheng Wen Yao1,
Ji Won Cha1,
Hee Kyoung Kang1,
Eun Sook Yoo1,
Young Sang Koh1,
Nam Ho Lee2,
Mi Hee Ko3,
Jin Won Hyun1 
For correspondence:- Jin Hyun Email: jinwonh@jejunu.ac.kr Tel:+82647022687
Received: 3 September 2013 Accepted: 6 April 2014 Published: 26 June 2014
Citation: Hyun YJ, Piao MJ, Kim KC, Zheng J, Yao CW, Cha JW, et al. Photoprotective Effect of a Polyopes affinis (Harvey) Kawaguchi and Wang (Halymeniaceae)-Derived Ethanol Extract on Human Keratinocytes. Trop J Pharm Res 2014; 13(6):863-871 doi: 10.4314/tjpr.v13i6.6
© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the photoprotective effect of the ethanol extract of the red marine alga, Polyopes affinis (PAE) against ultraviolet B (UVB) radiation on cultured human keratinocytes.
Methods: The 2',7'-dichlorodihydrofluorescein diacetate method was used to detect intracellular reactive oxygen species (ROS) generated by H2O2 treatment or UVB radiation. Cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ) assay. Superoxide anion or hydroxyl radical was detected using an electron spin resonance spectrometer after reaction with the nitrone spin trap. Lipid peroxidation was assayed by determining the level of 8-isoprostane. Protein carbonyl formation was determined using a protein carbonyl ELISA kit. The degree of oxidative DNA damage was determined using an alkaline comet assay. Apoptosis was assessed by apoptotic bodies and DNA fragmentation.
Results: PAE significantly scavenged the free radical 1,1-diphenyl-2-picrylhydrazyl, as well as hydrogen peroxide- and UVB-induced intracellular ROS. Furthermore, PAE showed 23 % scavenging effect of the superoxide anion and 33 % of the hydroxyl radical. PAE also absorbed UVB rays in the 280 – 320 nm range. PAE significantly decreased cellular damage resulting from UVB-induced oxidative stress to lipids, proteins, and DNA. Furthermore, PAE-treated keratinocytes showed significant reduction in UVB-induced apoptosis, as exemplified by fewer apoptotic bodies and reduced DNA fragmentation.
Conclusion: These results suggest that PAE protects keratinocytes against UVB-induced oxidative stress by absorbing UVB rays and scavenging ROS, thereby reducing injury to cellular constituents.
Archives
News Updates
Fulltext in PDF