Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Cytotoxic and Apoptotic Effect of the Decoction of the Aerial Parts of Flueggea leucopyrus on Human Endometrial Carcinoma (AN3CA) Cells

Sameera R Samarakoon¹ , Saman B Kotigala¹, Ishani Gammana-Liyanage¹, Ira Thabrew¹, Kamani H Tennekoon¹, Asitha Siriwardana², Prasanna B Galhena³

¹Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, 90, Cumarathunga Munidasa Mawatha, Colombo 3; ²Industrial Technology Institute, Bauddhaloka Mawatha, Colombo 07; ³Department of Biochemistry and Clinical Chemistry, Faculty of Medicine,University of Kelaniya, Thalagolla Road, Ragama, Sri Lanka.

For correspondence:- Sameera Samarakoon Email: sam@ibmbb.cmb.ac.lk Tel:+94112552528

Received: 19 February 2014 Accepted: 26 April 2014 Published: 26 June 2014

Citation: Samarakoon SR, Kotigala SB, Gammana-Liyanage I, Thabrew I, Tennekoon KH, Siriwardana A, et al. Cytotoxic and Apoptotic Effect of the Decoction of the Aerial Parts of Flueggea leucopyrus on Human Endometrial Carcinoma (AN3CA) Cells. Trop J Pharm Res 2014; 13(6):873-880 doi: 10.4314/tjpr.v13i6.7

© 2014 The authors.
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Abstract

Purpose: To evaluate the anti-cancer potentials of a decoction of Flueggea. leucopyrus (Willd.) on human endometrial carcinoma (AN3CA) cells.

Methods: Decoction was prepared by boiling 60 g of the ground plant material in 1.6 L of distilled water for about 3 h to reduce the volume to 200 mL and then freeze dried. The effect of the decoction on AN3CA cells was determined by evaluating its cytotoxicity by 3-(4, 5-dimethylthiazol-2yl) -2, 5-biphenyl tetrazolium bromide (MTT) and sulphorhodamine B (SRB) assays, as well as its ability to modulate apoptosis (microscopic observation of morphological changes, DNA fragmentation and caspase activity). The antioxidant activity of the decoction was also determined by DPPH assay, and its total polyphenolic and flavonoid content.

Results: The decoction exerted a significant dose-dependent cytotoxicity on AN3CA cells as evident from MTT assay IC₅₀ values of 22.09 and 14.60 μg/mL at 24 and 48 h post-incubation, respectively; and SRB assay IC₅₀ values of 28.60 and 15.09 μg/mL at 24 and 48 h post-incubation, respectively. The decoction also enhanced apoptosis as shown by enhanced DNA fragmentation, microscopic observation of nuclear condensation, fragmentation and apoptotic bodies and enhanced caspase 3 and 9 activities, as well as moderately increased radical scavenging activity.

Conclusion: The cytotoxic and apoptotic effects demonstrated by F. leucopyrus (Willd.) decoction provide supportive evidence for the ethnomedicinal use of this plant for cancer therapy.

Keywords: Fleuggea leucopyrus (Willd.), Endometrial carcinoma cells, Cytotoxicity, Apoptosis, Antioxidant, Anti-cancer