Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Interaction between Polymorphisms in Insulin Receptor Substrate Genes in Type 2 Diabetes Mellitus Patients with Severe/Acute Hyperglycemia

Hasniza Zaman Huri^1,2 , Mohd Makmor-Bakry³, Rosnani Hashim⁴, Norlaila Mustafa⁵, Wan Zurinah Wan Ngah⁶

¹Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; ²Clinical Investigation Centre, 13th Floor, Main Tower University of Malaya Medical Centre, 59100 Lembah Pantai, Kuala Lumpur, Malaysia; ³Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia; ⁴Faculty of Pharmacy, Cyberjaya University College of Medical Sciences Cyberjaya, Malaysia; ⁵Department of Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia; ⁶Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

For correspondence:- Hasniza Huri Email: hasnizazh@um.edu.my Tel:+60379676659

Received: 15 October 2013 Accepted: 21 March 2014 Published: 26 June 2014

Citation: Huri HZ, Makmor-Bakry M, Hashim R, Mustafa N, Ngah WZ. Effect of Interaction between Polymorphisms in Insulin Receptor Substrate Genes in Type 2 Diabetes Mellitus Patients with Severe/Acute Hyperglycemia. Trop J Pharm Res 2014; 13(6):889-894 doi: 10.4314/tjpr.v13i6.9

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate whether there are interactions between insulin receptor substrate 1 (IRS1) and insulin receptor substrate 2 (IRS2) that are associated with increased insulin resistance during such episodes.

Methods: Testing Haplotype EffectS in Association Studies (THESIAS) software was used to investigate allelic and haplotype interactions between the polymorphisms in 156 T2DM patients with severe or acute hyperglycemia.

Results: Binary analysis showed there were significant differences in the haplotype frequencies for the IRS1 and IRS2 polymorphisms based on the insulin resistance status. Nevertheless, estimation of haplotype effects by equality analysis showed no significant interactions (likelihood ratio tests: all p > 0.05) in increased insulin resistance in T2DM patients with severe/acute hyperglycemia.

Conclusion: There are no interactions between IRS1 rs1801278 (p.Gly972Arg) and IRS2 rs1805097 (p.Gly1057Asp) polymorphisms that would affect insulin resistance in T2DM patients with severe/acute hyperglycemia.

Keywords: Insulin receptor substrate, Proteins, Insulin resistance, Diabetes mellitus, Hyperglycemia, Haplotype, Genetic polymorphism