Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Essential Oil Composition and Anti-Inflammatory Activity of Salvia officinalis L (Lamiaceae) in Murin Macrophages

Alev Tosun¹ , Salman Khan², Yeong Shik Kim², Ângel Calín-Sánchez³, Xhevit Hysenaj⁴, Ângel Antonio Carbonell-Barrachina³

¹Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, 06100, Tandoğan, Ankara, Turkey; ²Natural Product Research Institute, College of Pharmacy, Seoul National University, Seoul, 151-742, South Korea; ³Departamento TecnologÃa Agroalimentaria, Universidad Miguel HernÃ¡ndez de Elche, Carretera de Beniel, 03312 Orihuela, Alicante, Spain; ⁴Xherdo Ltd., Medicinal Plants and Essential Oils, Nikolla Lena, No.138, Tirana, Albania.

For correspondence:- Alev Tosun Email: pharmacogalev@gmail.com Tel:+903122033089

Received: 13 May 2013 Accepted: 2 April 2014 Published: 26 June 2014

Citation: Tosun A, Khan S, Kim YS, Calín-Sánchez &, Hysenaj X, Carbonell-Barrachina &A. Essential Oil Composition and Anti-Inflammatory Activity of Salvia officinalis L (Lamiaceae) in Murin Macrophages. Trop J Pharm Res 2014; 13(6):937-942 doi: 10.4314/tjpr.v13i6.16

© 2014 The authors.
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Abstract

Purpose: Sage, Salvia officinalis L (Lamiaceae), is widely cultivated medicinal plant for its economic importance and large content of bioactive components; therefore, in the present study, the active components (volatile compounds) and the anti-inflammatory effect of S. officinalis have been investigated.

Methods: Salvia officinalis samples were collected from northern and southern Albania, respectively. The crushed leaves were subjected to hydro-distillation, and the essential oils analyzed by gas chromatography GC/FID (for quantification of volatiles) and gas chromatography/mass spectroscopy (GC/MS) for identification.

Results: α-Thujone (30.7 %), camphor (26.6 %) and 1, 8-cineole (14.7 %) were the major components of the oil from northern Albania, while camphor (43.8 %), α-thujone (15.9 %), camphene (8.5 %) and 1,8-cineole (8.4 %) were the predominant compounds in the sample from southern Albania. The results of the anti-inflammatory tests on these essential oils using murine macrophages indicate that both oils significantly (p < 0.05) reduced nitric oxide (NO), and nuclear kappa B (NF-κB) production in RAW 264.7 cells.

Conclusion: The results indicate that NO and NF-κB production in RAW 264.7 cells are greatly decreased by the essential oil of Albanian sage. Thus, the biological properties of sage oil can be attributed to the components of the oil.

Keywords: Salvia officinalis, Sage oil, Camphor, Camphene, Lamiaceae, Cineole, Nuclear kappa B, Nitric oxide, ^5;-Thujone, Volatile compound