Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Total Antioxidant Activity, and Hesperidin, Diosmin, Eriocitrin and Quercetin Contents of Various Lemon Juices

Mannan Hajimahmoodi¹ , Ghazaleh Moghaddam¹, Seyed Mohsen Mousavi¹, Naficeh Sadeghi¹, Mohammad Reza Oveisi¹, Behrooz Jannat²

¹Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences; ²Drug and Food Control Laboratory, Research Center, Ministry of Health and Medical Education, Tehran, Iran.

For correspondence:- Mannan Hajimahmoodi Email: hajimah@sina.tums.ac.ir Tel:+982166954714

Received: 12 June 2013 Accepted: 27 March 2014 Published: 26 June 2014

Citation: Hajimahmoodi M, Moghaddam G, Mousavi SM, Sadeghi N, Oveisi MR, Jannat B. Total Antioxidant Activity, and Hesperidin, Diosmin, Eriocitrin and Quercetin Contents of Various Lemon Juices. Trop J Pharm Res 2014; 13(6):951-956 doi: 10.4314/tjpr.v13i6.18

© 2014 The authors.
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Abstract

Purpose: To investigate 38 natural and 62 branded lemon juices for their total antioxidant activities and flavonoid content.

Methods: The ferric reducing antioxidant power was applied to evaluation of antioxidant. Additionally quercetin, eriocitrin, diosmin and hesperidin contents were evaluated by high performance liquid chromatography (HPLC).

Results: Total antioxidant activity of the juices ranged from 91.38 ± 0.01 to 526.93 ± 0.12 µg/ml. The maximum contents of quercetin, hesperidin and eriocitrin 14.83 ± 2.83, 104.84 ± 12.58 and 10.68 ± 2.41 µg/mL, respectively. The natural juices had diosmin levels as high as 236.18 mL. The limit of detection (LOD) ranged from 0.15 µg mg-1 for hesperidine to 0.53 µg ml-1 for diosmin while the limit of quantification (LOQ) ranged from 0.27 µg mg-1 for hesperidine to 0.93 µg ml-1 for quercetin.

Conclusion: The developed method can also be applied to the determination of related extracts of orange cultivars as well as various extracts of medicinal plants.

Keywords: Antioxidant, Flavonoid, Hesperidin, Diosmin, Eriocitrin, Quercetin, Lemon juice