Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Simultaneous Determination of Ciprofloxacin and Tinidazole in Tablet Dosage Form by Reverse Phase High Performance Liquid Chromatography

T Sirisha¹ , BM Gurupadayya¹, S Sridhar²

¹Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysore; ²Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Secunderabad, India.

For correspondence:- Sirisha Email: tadiboyina.sirisha@gmail.com Tel:+8884699984

Received: 30 August 2013 Accepted: 24 March 2014 Published: 26 June 2014

Citation: Sirisha T, Gurupadayya B, Sridhar S. Simultaneous Determination of Ciprofloxacin and Tinidazole in Tablet Dosage Form by Reverse Phase High Performance Liquid Chromatography. Trop J Pharm Res 2014; 13(6):981-987 doi: 10.4314/tjpr.v13i6.23

© 2014 The authors.
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Abstract

Purpose: To develop a simple, sensitive and specific liquid chromatographic method with PDA detection for the simultaneous estimation of ciprofloxacin and tinidazole in tablet dosage form.

Methods: Separation was achieved with an Agilent XDB C18, 250 × 4.60 mm 5 µ column, low pressure gradient mode with a ambient temperature and mobile phase comprising acetonitrile water containing 0.1 % orthophosphoric (20:80). The flow rate was 1 ml/min and eluent was monitored spectrophotometrically at 316 nm.

Results: The selected chromatographic conditions effectively separated ciprofloxacin and tinidazole with retention time of 3.036 and 4.224 min, respectively. Linearity for ciprofloxacin and tinidazole was in the range 50 - 100 and 60 - 120 µg/ml, respectively. Regression coefficient was 0.999 for both ciprofloxain and tinidazole while recovery waas 100.19 - 100.92 and 99.36 - 100.48 % for ciprofloxacin and tinidazole, respectively. Relative standard deviation (RSD) of intra- and inter-day precision was < 2 % for both drugs.

Conclusion: The developed method is precise, accurate, reproducible and specific and it can also be used for routine simultaneous quality-control analysis of ciprofloxacin and tinidazole in combination tablets.

Keywords: High performance liquid chromatography, Ciprofloxacin, Tinidazole, Simultaneous determination