Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Formulation of Thermosensitive Hydrogel Containing Cyclodextrin for Controlled Drug Delivery of Camptothecin

Swatantra Kumar Singh Kushwaha¹ , Awani Kumar Rai¹, Satyawan Singh²

¹Pranveer Singh Institute of Technology, Kanpur; ²Saroj Institute of Technology & Management, Lucknow, India.

For correspondence:- Swatantra Kushwaha Email: swatantrakushwaha@yahoo.co.in Tel:+919450560321

Received: 30 August 2013 Accepted: 1 May 2014 Published: 25 July 2014

Citation: Kushwaha SK, Rai AK, Singh S. Formulation of Thermosensitive Hydrogel Containing Cyclodextrin for Controlled Drug Delivery of Camptothecin. Trop J Pharm Res 2014; 13(7):1007-1012 doi: 10.4314/tjpr.v13i7.1

© 2014 The authors.
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Abstract

Purpose: To formulate and evaluate temperature-sensitive, controlled-release camptothecin hydrogel for anticancer drug delivery.

Method: Temperature-sensitive hydrogel based on chitosan/β-glycerophosphate (β-GP)/β-cyclodextrin (β-CD) was prepared by crosslinking method. The formulations were characterized by Fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), gelation time, and viscometry, as well as for controlled release. The formulation, containing camptothecin, was studied by MTT assay on tumor cell MCF-7. The effectiveness of treatment was measured in terms of controlled tumor growth inhibition (TGI).

Results: The hydrogel formulation showed good properties in terms of pH, gelation, viscosity and in-vitro release. The gelation temperature and viscosity of the formulation was optimum. Camptothecin (CPT) released from the hydrogel (TF8) over 8 h in pH 7.4 buffer ranged from 38.97 - 92.5 %, and varied according to the composition of the hydrogels. Release of camptothecin was lowest from preparations without cyclodextrins. Tumor growth inhibition activity of CPT in MCF-7 cell was highest for the formulation containing 1 % chitosan, 8 % β-GP and 1 % β-CD while no inhibition was observed for the blank temperature sensitive hydrogel formulation.

Conclusion: These formulations are a promising and more effective delivery system that can be developed to serve as an alternative to the conventional system for anticancer drug delivery.

Keywords: Hydrogel, Chitosan, ^6;-Glycerophosphate, ^6;-Cyclodextrin, Camptothecin, MCF-7 cell line