Sohee Shin1,
Liu-Xiang Wang1,
Xin-Qiang Zheng1,
Li-Ping Xiang2,
Yue-Rong Liang1 
For correspondence:- Yue-Rong Liang Email: yrliang@zju.edu.cn Tel:+8657188982704
Received: 12 February 2014 Accepted: 2 June 2014 Published: 25 July 2014
Citation: Shin S, Wang L, Zheng X, Xiang L, Liang Y. Protective Effect of (-)-Epigallocatechin Gallate against Photo-Damage Induced by Ultraviolet A in Human Skin Fibroblasts. Trop J Pharm Res 2014; 13(7):1079-1084 doi: 10.4314/tjpr.v13i7.10
© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the photoprotective effect of (-)-epigallocatechin gallate (EGCG), one of tea catechins, on human skin fibroblast (HSF) irradiated by ultraviolet A.
Methods: HSF cells were incubated in serum-free Dulbecco's Modified Eagle's Medium (DMEM) with or without EGCG for 2 h, and then irradiated by UV A. Blank (control) was incubated in DMEM without EGCG and UV A-irradiation. Cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method. Protein concentration of the samples was determined using a PA102 Bradford protein assay kit. Malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide anion radicals were determined using MDA assay kit, GSH-Px assay kit and superoxide anion radical assay kit, respectively.
Results: HSF viability decreased with dosage of UV A irradiation with 50 % lethal dose A288;LD50A289;of 9 J/cm2. Pre-incubation of HSF in 10 μg/mL EGCG aqueous solution for 2 h before exposure to UV A alleviated the suppressive effect of UV A on HSF. Compared to UVA irradiation alone, HSF viability and GSH-Px activity in the EGCG pretreatment increased by 18.3 and 103.4 %, accompanying decrease in level of superoxide anion radicals and MDA by 44.6 and 16.6 %, respectively.
Conclusion: EGCG alleviates UV A-induced HSF photo-damage through relieving oxidative stress by increasing activity of GSH-Px and scavenging capacity of superoxide anion radical.
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