Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Influence of Extraction Parameters on Hydroalcohol Extracts of the Stem Bark of Rapanea ferruginea Mez Using Myrsinoic Acid B as Marker

Thaisa Baccarin¹, Rene A Ferreira¹, Vanessa F Gazoni¹, Rosendo Yunes², Ângela Malheiros¹, Ruth M Lucinda-Silva¹

¹NÃºcleo de InvestigaÃ§Ã£o QuÃmico-FarmacÃªutica (NIQFAR), Programa de Mestrado em CiÃªncias FarmacÃªuticas, Universidade do Vale do ItajaÃ, ItajaÃ-SC; ²Departamento de QuÃmica, Universidade Federal de Santa Catarina, FlorianÃ³polis-SC, Brazil.

For correspondence:- Ruth Lucinda-Silva Email: rlucinda@univali.br Tel:+554733417664

Received: 6 April 2013 Accepted: 1 May 2014 Published: 25 July 2014

Citation: Baccarin T, Ferreira RA, Gazoni VF, Yunes R, Malheiros &, Lucinda-Silva RM. Influence of Extraction Parameters on Hydroalcohol Extracts of the Stem Bark of Rapanea ferruginea Mez Using Myrsinoic Acid B as Marker. Trop J Pharm Res 2014; 13(7):1113-1119 doi: 10.4314/tjpr.v13i7.15

© 2014 The authors.
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Abstract

Purpose: To evaluate the influence of extraction parameters on standardization of hydroalcoholic extract of Rapanea ferruginea Mez.

Methods: A 3³ factorial design was used to evaluate the influence of alcohol concentration (50, 70 and 90 % v/v), extraction time (2, 6 and 10 h), and particle size of the herbal drug (0.25, 0.5 and 1.0 mm) on the pH, dry residue and myrsinoic acid B (MAB) content of hydroalcoholic extracts by high performance liquid chromatography (HPLC) method.

Results: For the extracts, there was inverse correlation between dry residue and MAB content (p < 0.8046). The parameter that had the greatest influence on extraction process was alcohol concentration (p < 0.007) followed by particle size (p < 0.046). However, extraction time did not influence the process in terms of MAB (p < 0.675). Although, the highest MAB content was obtained with 70 % v/v alcohol and extraction time of 6 h, the best methodology for extractive preparation was the use of 90 % v/v alcohol and extraction time of 2 h because it resulted in a higher yield of MAB per solution volume, i.e,, with regard to MAB content and mass yield.

Conclusion: The results indicate that 90 % v/v alcohol and extraction time of 2 h have optimum influence on extraction process and were the best conditions for obtaining a standardized extract of R. ferruginea, using the method employed in this study. Thus, the findings of this study are a contribution to the development of a new phytopharmaceutical intermediate product.

Keywords: Rapanea ferruginea, Hydroalcoholic extract, Myrsinoic acid B, Phytopharmaceutical, Factorial desing, Standardization