Wei Cai1,
Jia Yu Zhang2,
Guang Lei Li1,
Guo Ping Chang1,
Ya Lei Wei1,
Hong Gui Zhang1 
,
Jian Qiu Lu2
For correspondence:- Jian Lu Email: lujq@vip.sina.com Tel:+86 10 84738642
Received: 9 July 2014 Accepted: 5 November 2014 Published: 15 December 2014
Citation: Cai W, Zhang JY, Li GL, Chang GP, Wei YL, Zhang HG, et al. Isolation and Purification of Sesquiterpene Lactones from Ixeris sonchifolia (Bunge) Hance by High-Speed Counter-Current Chromatography and Semi-Preparative High Performance Liquid Chromatography. Trop J Pharm Res 2014; 13(12):2065-2069 doi: 10.4314/tjpr.v13i12.17
© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To isolate and purify sesquiterpene lactones from Ixeris sonchifolia (Bunge) Hance by high-speed counter-current chromatography (HSCCC).
Methods: I. sonchifolia was extracted with water and then loaded on a glass column (10 × 1500 cm containing 3000g D101 macroporous resin) where various concentrations of aqueous ethanol (0, 10, 30, 50, and 95 %) were used to elute the column successively. The 50 % ethanol fraction was purified by HSCCC using a solvent system comprised of ethyl acetate: n-butanol: methanol: water (4: 6: 1: 20, v/v), and semi-preparative high performance liquid chromatography (HPLC). The chemical structures of the components obtained were further confirmed by high-resolution mass spectroscopy (MS) and nuclear magnetic resonance spectroscopy (NMR).
Results: Three compounds, including ixerin Z1 (0.7 mg), ixerin Z (11.4 mg), and 11, 13α-dihydroixerin Z (8.2 mg), with purity of 96.2, 98.2, and 98.4 %, respectively, were obtained from 200 mg each of the 50 % ethanol fraction.
Conclusion: HSCCC is a rapid and effective method for isolating and purifying sesquiterpene lactones from I. sonchifolia.
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