Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of the Antioxidant Activities of Ya-hom Intajak, a Thai Herbal Formulation, and its Component Plants

Jantanarak Tuekaew¹, Nisarat Siriwatanametanon¹, Yuvadee Wongkrajang², Rungravi Temsiririrkkul¹, Ibrahim Jantan³

¹Department of Pharmaceutical Botany; ²Department of Physiology, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road, Rajathevi, Bangkok, 10400, Thailand; ³Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.

For correspondence:- Ibrahim Jantan Email: profibj@gmail.com Tel:+60392897315

Received: 25 February 2014 Accepted: 3 August 2014 Published: 24 September 2014

Citation: Tuekaew J, Siriwatanametanon N, Wongkrajang Y, Temsiririrkkul R, Jantan I. Evaluation of the Antioxidant Activities of Ya-hom Intajak, a Thai Herbal Formulation, and its Component Plants. Trop J Pharm Res 2014; 13(9):1477-1485 doi: 10.4314/tjpr.v13i9.14

© 2014 The authors.
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Abstract

Purpose: To evaluate the antioxidant effect of 80 % ethanol extract and its n-hexane and dichloromethane fractions of Ya-hom Intajak, and its 47 medicinal plants components.

Methods: The 80 % ethanol extract and its n-hexane and dichloromethane fractions were investigated on human low-density lipoprotein (LDL) peroxidation by thiobarbituric acid reactive substances (TBARS) assay. Antioxidant effect was also determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and ferric reducing antioxidant power (FRAP) assays.

Results: The extract of Ya-hom Intajak exhibited moderate DPPH scavenging activity with an IC₅₀ value of 99.08 μg/ml and a FRAP value of 1.12 mmol FeSO4/g. Among the individual plants, Terminalia chebula Retz., Caesalpinia sappan L., Cinnamomum bejolghota (Buch.-Ham.) and Ci. verum J. Presl, showed strong antioxidant activities. T. chebula was the most potent plant in the DPPH assay while Ca. sappan had the highest FRAP value. Among the extracts, the dichloromethane fraction showed the highest inhibitory effect on LDL peroxidation with a half maximal inhibitory concentration (IC₅₀) value comparable to that of probucol (0.82 μg/ml). Pearson correlation analysis revealed that total phenolic content (TPC) showed high positive correlations with FRAP (r = 0.908, p < 0.01) and DPPH activities (r = 0.648, p < 0.01).

Conclusion: Ya-hom Intajak is a valuable source of natural antioxidants and has a potential application as a cardiovascular protective formulation.

Keywords: Ya-hom Intajak, Antioxidant activity, Terminalia chebula Retz., Caesalpinia sappan L., Cinnamomum bejolghota (Buch.-Ham.), Ci. verum J. Presl , Phenol