Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-Hyperlipidemic Effect of Flavone-Rich Belamcanda chinensis (L.) DC. (Iridaceae) Leaf Extract in ICR Mice Fed High-Fat Diet

Hai-Wei Zhao¹, Fang Lv^1,2, Wei-Wei Meng^1,2, Hao Dang¹, Zhi-Long Sun¹, Yan Chen^1,2, Rong-Ji Dai^1,2, Yu-Lin Deng^1,2, Chong-Ming Wu³

¹School of Life Science, Beijing Institute of Technology, Beijing 100081; ²Beijing BIT&GY Pharmaceutical R&D, Beijing 100081; ³Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, PR China.

For correspondence:- Chong-Ming Wu Email: wucm1979@gmail.com Tel:+861068949331

Received: 24 July 2013 Accepted: 24 August 2014 Published: 19 October 2014

Citation: Zhao H, Lv F, Meng W, Dang H, Sun Z, Chen Y, et al. Anti-Hyperlipidemic Effect of Flavone-Rich Belamcanda chinensis (L.) DC. (Iridaceae) Leaf Extract in ICR Mice Fed High-Fat Diet. Trop J Pharm Res 2014; 13(10):1653-1658 doi: 10.4314/tjpr.v13i10.12

© 2014 The authors.
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Abstract

Purpose: To assess the anti-hyperlidemic effect of flavone-rich B. chinensis leaf extract (HTP) in ICR mice fed a high-fat diet.

Methods: HTP (100, 200 and 400 mg/kg) were orally administered to ICR mice fed high-fat diet for 7 weeks. Blood glucose, as well as serum and hepatic levels of lipids were determined at the end of the experiment. Phosphorylation of adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) and protein level of peroxisome proliferator-activated receptor α (PPARα) were analyzed by Western blot and enzyme-linked immunosorbent assay (ELISA) kit, respectively.

Results: Treatment with HTP significantly decreased peri-epididymal fat weight (p < 0.01 and p < 0.05 for 200 and 100 mg/kg, respectively), lowered serum and hepatic lipid, and decreased glucose area under curve (AUC) in oral glucose tolerance test (p < 0.01 for 200 mg/kg). Western blot and ELISA analysis showed that administration of HTP (200 mg/kg) significantly increased AMPK (p < 0.05) phosphorylation and PPARα expression in liver (p < 0.05).

Conclusion: HTP can alleviate hyperlipidemia, at least in part, by up-regulation of AMPK and PPARα.

Keywords: Belamcanda chinensis, Flavone, Hyperlipidemia, Adenosine 5R42;-monophosphate-activated protein kinase, Peroxisome proliferator activated receptor-alph