Yujie Liu1,
Ying Xu1,
Huaiyao Qiao2,
Xiao Ke2,
Zhigang Wu2,
Tao Sun1,
Chunjie Wu1 
For correspondence:- Chunjie Wu Email: wuchunjie@gmail.com
Received: 18 June 2014 Accepted: 11 September 2014 Published: 19 October 2014
Citation: Liu Y, Xu Y, Qiao H, Ke X, Wu Z, Sun T, et al. Online Monitoring for Strychnos Nux-vomica Parching in Sands and Chemical Compositional Analysis by Ultra Performance Liquid Chromatography - Linear Trap Quadrupole -Orbitrap- Mass Spectrometry. Trop J Pharm Res 2014; 13(10):1675-1680 doi: 10.4314/tjpr.v13i10.15
© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To provide an online and non-contact method to monitor the dynamic temperature of the nux-vomica parching in sands, and also to develop a rapid screening and comparison of the compounds in crude and processed nux-vomica.
Methods: Seeds of nux-vomica were parched in sands by an herbal medicine roaster with an online-type and non-contact temperature measurement system (ONTMS). Processing was monitored by ONTMS and the processing temperature was accurately controlled within the range 200 - 220 oC. Ultra performance liquid chromatography - linear trap quadrupole -orbitrap-mass spectrometry method in positive ionization mode was established in this work. The samples were extracted by ultrasonication with 50 % methanol for 30 min. Chromatographic separation was achieved on an Acquity UPLC HSS T3 column using a gradient program with acetonitrile and 1 % formic acid as the mobile phase and a diode array detector set at 254 nm for quantification.
Results: The surface color of the processed seeds of nux-vomica were brown or dark brown and the cross-section bulged with small bubble which meets the requirements of Chinese traditional processing methods. Fourteen major compounds from crude and processed nux-vomica were identified simultaneously by HPLC-DAD. According to the MS fragmentation pathways and by comparing with reference literature, the structures of eleven of the compounds were confirmed.
Conclusion: Methods using ONTMS are more accurate for parching nux-vomica in sands and UPLC-LTQ-Orbitrap-MS is a rapid and straightforward method for compound profiling in crude and processed nux-vomica.
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