Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of nutrient formulations on permeation of proteins and lipids through porcine intestine n vitro

Prasopchai Tonglairoum, Praneet Opanasopit

Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand;

For correspondence:- Praneet Opanasopit Email: praneet@su.ac.th

Received: 24 September 2014 Accepted: 2 May 2015 Published: 29 July 2015

Citation: Tonglairoum P, Opanasopit P. Effect of nutrient formulations on permeation of proteins and lipids through porcine intestine n vitro. Trop J Pharm Res 2015; 14(7):1161-1166 doi: 10.4314/tjpr.v14i7.6

© 2015 The authors.
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Abstract

Purpose: To investigate the effect of nutrient formulations on the permeation of proteins and lipids through porcine intestine in vitro.

Methods: In vitro permeation studies of proteins and lipids of two peptide-based formulations, composed of various compounds and sources of hydrolyzed protein was carried out, and compared with a conservative polymeric formulation as control, The test was undertaken using Franz diffusion cell apparatus incorporating porcine intestine.

Results: The peptide-based formulation demonstrated higher protein absorption than the conservative polymeric one. However, there were some differences in protein absorption rates between the peptide-based formulations obtained from various sources. Formulation A with 1.0 and 1.5 kcal/mL exhibited significantly (p < 0.05) higher cumulative protein permeation (11.97 ± 0.23 and 12.54 ± 0.94 µg/cm²) than formulations B (9.41 ± 0.36 and 9.67 ± 0.35 µg/cm²) and C (8.34 ± 0.56 and 8.61 ± 0.71 µg/cm²), respectively. Lipid permeation from formulations A and B (13.91 ± 0.26 and 12.94 ± 0.59 µg/cm² respectively for 1.0 kcal/mL formulation, and 13.31 ± 0.21 and 12.86 ± 0.16 for 1.5 kcal/mL formulation) which consist mainly of medium chain triglycerides (MCTs), were significantly (p < 0.05) higher than those from formulation C (11.49 ± 0.43 and 12.62 ± 0.38 µg/cm² for 1.0 and 1.5 kcal/mL formulation, respectively) which mostly contained long chain triglycerides (LCTs).

Conclusion: The results reveal that oligomeric formulations have higher absorption rate than polymeric formulations. However, the outcomes when administered to clinically ill patients need to be investigated.

Keywords: Nutrient formulations, Permeation, Proteins, Lipids, Porcine intestine, Medium chain triglycerides, Long chain triglycerides