Xiaoli Wang1,
Fan Wang2,
Yanjun Jing2,
Yonggang Wang2,
Peng Lin2,
Lin Yang2 
,
For correspondence:- Lin Yang Email: 412316788@163.com Tel:+86309312976707
Received: 30 December 2014 Accepted: 5 June 2015 Published: 29 July 2015
Citation: Wang X, Wang F, Jing Y, Wang Y, Lin P, Yang L, Application of orthogonal design to optimize extraction of polysaccharide from Cynomorium songaricum Rupr (Cynomoriaceae). Trop J Pharm Res 2015; 14(7):1175-1181 doi: 10.4314/tjpr.v14i7.8
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To optimize the extraction technology of polysaccharides from Cynomorium songaricum Rupr by ultrasonic-assisted extraction (UAE).
Methods: Four parameters including ultrasonic power, ratio of raw material to water, extraction temperature, and extraction time were optimized by orthogonal design. The effects of the factors on the yield of polysaccharides were also studied. The hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were determined in vitro by spectrophotometry.
Results: The optimal conditions were as follows: 1:30 as ratio of raw material to water, extraction for 70 min at 80 oC with ultrasonic power being 420 W. Under these conditions, the yield of polysaccharides was up to 4.51 %, which was significantly higher than that obtained under the initial conditions (3.82 %). DPPH radical scavenging activity reached 64.82 % at 0.012 mg/mL, while hydroxyl radical scavenging activity was 18.36 % at 0.5 mg/mL.
Conclusion: Ultrasonic-assisted extraction technology is a useful tool for the extraction of bioactive components from biological materials.
Archives
News Updates
Fulltext in PDF