Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of histone deacetylase inhibitor, trichostatin A, on cartilage regeneration from free perichondrial grafts in rabbits

Ning Zeng, Haiping Wang, Yi Xu, Yiping Wu, Min Wu

Department of Plastics and Aesthetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030, China;

For correspondence:- Min Wu Email: minwu1973@hotmail.com Tel:+862783663568

Received: 6 January 2017 Accepted: 16 May 2017 Published: 29 June 2017

Citation: Zeng N, Wang H, Xu Y, Wu Y, Wu M. Effect of histone deacetylase inhibitor, trichostatin A, on cartilage regeneration from free perichondrial grafts in rabbits. Trop J Pharm Res 2017; 16(6):1253-1257 doi: 10.4314/tjpr.v16i6.7

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the effect of histone deacetylase (HDAC) inhibitor, trichostatin A (TCA), on cartilage regeneration in a rabbit perichondrial graft model.

Methods: Perichondrial grafts (20 × 20 mm2) were derived from the ears of New Zealand rabbits and transplanted onto the paravertebral muscle of the face of each rabbit. The rabbits were separated into three groups: non-treated control group, vehicle-treated control group that received 0.3 mL of saline, and TCA-treated group administered 0.3 mL of TCA (500 ng/mL). Rabbits in all three groups were further divided into subgroups according to the duration of treatment after transplantation: 2, 4, 6, and 8 weeks (n = 12 rabbits each). The effect of TCA on cartilage regeneration was determined histologically by evaluating the thickness of the cartilage plate in the grafted rabbits.

Results: TCA increased the amount of immature cartilage 4 and 6 weeks after perichondrial graft implantation. Mature cartilage was seen in the TCA-treated rabbits 8 weeks after transplantation. The thickness of the cartilage plate was significantly (p < 0.01) higher in TCA group (905 ± 36) than in either the non-treated (632 ± 22) or the vehicle-treated control (639 ± 22) group.

Conclusion: Treatment with trichostatin A, an HDAC inhibitor, enhances cartilage regeneration in rabbit recipients of a perichondrial graft. Furthermore, the findings of this study should be helpful in exploring the clinical use of trichostatin A.

Keywords: Histone deacetylase inhibitor, Perichondrial graft, TrichostatinA, Cartilage regeneration, Transplantation