Gongbo Liu1,
Qingtao Li2,
Wenping Wang1,
Junzi Jiang1,
Jiangui Liao1,
Liangpeng Ge3,
Chuan Cao3,
Shirong Li1 
For correspondence:- Shirong Li Email: shironglicq@163.com
Received: 27 April 2017 Accepted: 28 June 2017 Published: 31 July 2017
Citation: Liu G, Li Q, Wang W, Jiang J, Liao J, Ge L, et al. Formulation and characterization of a novel, photo-initiated small intestinal sub-mucosal wound-healing hydrogel. Trop J Pharm Res 2017; 16(7):1473-1480 doi: 10.4314/tjpr.v16i7.3
© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To design and characterize a novel 3-D photo-initiated small intestinal sub-mucosal (SIS) hydrogel for use as a scaffold.
Methods: Two concentrations of hydrogel were used: 10 mg/mL SIS gel (designated as 1 % hydrogel) and 20 mg/mL SIS gel (designated as 2 % hydrogel). Cross-sections of the hydrogels were examined by scanning electron microscope. In vitro cell culture was carried out on the hydrogels, and cell count was obtained on each hydrogel at different time points. In addition, hematoxylin-eosin (H&E) staining was used to assess in vivo biodegradability of the gels, as well as tissue regeneration.
Results: The 1 % hydrogel possessed a larger pore size (143 ± 22 μm) than the 2 % hydrogel (113 ± 17 μm) and showed significantly higher biodegradation rate (22.79 ± 2.47 % of gel left on day 5) than 2% hydrogel (35.37 ± 4.51 % of gel left on day 5) (p < 0.05). However, results from cell culture showed that the 2 % hydrogel had better biocompatibility than 1 % hydrogel. In vivo data revealed that the gels supported cell growth (cell count on days 3 and 5 were 48.33 ± 17.61 and 105.67 ± 21.36, respectively).
Conclusion: These results suggest that SIS hydrogels have a high potential for application in tissue regeneration.
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