Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Leucine zipper transcription factor-like 1 expression in gastric cancer and its relationship to relative adhesion molecule

Lian Xiaowen^1,2, Li Kesheng², Du Huifen², Li Xingwen³, Cui Yan , Chai Dandan³, Xu Yang², Yang Rong²

¹College of Veterinary Medicine, Gansu Agricultural University; ²Department of Medicine Biotechnology, Medicine and Science Research Institute; ³Department of Surgery, Tumor Hospital of Gansu Province; ⁴Department of Pathology Gansu Provincial Tumor Hospital.

For correspondence:- Cui Yan Email: cuiyangau@163.com

Received: 14 March 2017 Accepted: 26 June 2017 Published: 31 July 2017

Citation: Xiaowen L, Kesheng L, Huifen D, Xingwen L, Yan C, Dandan C, et al. Leucine zipper transcription factor-like 1 expression in gastric cancer and its relationship to relative adhesion molecule. Trop J Pharm Res 2017; 16(7):1537-1544 doi: 10.4314/tjpr.v16i7.11

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the expression of leucine zipper transcription factor-like1 (LZTFL1) molecule in the gastric cancer tissues, and its relationship to cellular adhesion protein.

Methods: expressions of LZTFLl, E-cadherin, β-catenin, intercellular cell adhesion molecule-1(ICAM-1) as well as vascular cell adhesion molecule-1 (VCAM-1) in the 133 gastric cancer samples and 40 gastritis samples were evaluated by immunohistochemistry. Levels of mRNA in gastric cancer and latero-cancer tissue were determined by real-time polymerase chain reaction (RT-PCR).

Results: Protein levels in gastric tissue decreased. The expression of LZTFL1in gastric cancer tissue correlated with tumor stage (tumor node metastasis staging), degree of tumor differentiation and invasion depth (p < 0.05). Protein expression correlated with E-cadherin positively, and correlated with β-catenin and ICAM-1 negatively, but had no correlation with VCAM-1. Compared with tissues remote to the cancer, mRNA level of LZTFL1 in gastric cancer decreased significantly. There was no significant difference in LZTFL1 mRNA levels in the various clinical pathological tissues. LZTFL1 mRNA expression did not correlation with various adhesion factors.

Conclusion: LZTFL1 is expressed at a low level in gastric cancer tissue. Its protein expression is related to cellular adhesion protein, but not to mRNA level. As a suppressor of invasion of cancer cells, LZTFL1 may be a potential target for targeted therapy of gastric cancer.

Keywords: Leucine zipper transcription factor-like1 expression, Gastric cancer, Relative adhesion molecule, Protein expression