Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Comparative analysis of hyoscine in wild-type and in vitro-grown Datura innoxia by high performance liquid chromatography

Sidra Siddiqui, Anam Khurshid, Sohaib Roomi, Fazeelat Karamat, Asrar Muhammad Khan, Humaira Shaheen, Tayyaba Yasmin

Department of Biosciences, COMSATS Institute of Information Technology, 54440 Tarlaikalan, Islamabad, Pakistan;

For correspondence:- Tayyaba Yasmin Email: tayyaba_yasmin@comsats.edu.pk

Received: 16 December 2016 Accepted: 3 June 2017 Published: 31 July 2017

Citation: Siddiqui S, Khurshid A, Roomi S, Karamat F, Khan AM, Shaheen H, et al. Comparative analysis of hyoscine in wild-type and in vitro-grown Datura innoxia by high performance liquid chromatography. Trop J Pharm Res 2017; 16(7):1683-1692 doi: 10.4314/tjpr.v16i7.29

© 2017 The authors.
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Abstract

Purpose: To compare the hyoscine contents of Datura innoxia plant grown in the wild and that grown in vitro.

Methods: For callus induction from leaf explants, 15 combinations of 2,4-dichlorophenoxy acetic acid, gibberellic acid, benzyl amino purine and kinetin were used. For regeneration of shoots, 8 combinations of BAP, kinetin and indole butyric acid (IBA) were tested. Different concentrations of IBA were used for rooting. The hyoscine contents of the wild and in vitro samples was initially identified by TLC and subsequently quantified by high performance liquid chromatography using hyoscine N-butyl bromide as standard.

Results: For callus induction, 2,4-D at 2 mg/L produced maximum calli (96 %). Maximum shoots regeneration was obtained with 2 mg/L BAP and 1 mg/L kinetin. IBA at 0.5 mg/L induced good rooting. Substantial amount of hyoscine was detected in the extracts of in vitro grown plants through TLC and HPLC. Wild root, stem and leaves exhibited higher amounts (approx. 2 µg/mL) of hyoscine than the parts of in vitro grown plants. Green callus grown in vitro contained a maximum concentration of hyoscine (1.01 µg/mL) followed by leaf (0.82 µg/mL) and brown callus (0.432 µg/mL).

Conclusion: The callus cultures of D. innoxia are capable of yielding good amounts of hyoscine, and therefore can be exploited to increase hyoscine production using chemical/hormone treatments on a large scale. Thus, this study provides a sustainable and efficient way for the sustainable production of a natural hyoscine product via in vitro-grown D. innoxia cultures.

Keywords: Datura innoxia, Hyoscine, Callus, Hyoscine, Explant, Gibberellic acid, Benzyl amino purine, Kinetin