Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro evaluation of mutagenicity and genotoxicity of sitagliptin alone and in combination with artificial sweeteners

Komal Najam^1,2, Imran Altaf³, M Ashraf¹, M Adil Rasheed¹, Faiza Saleem⁴, Neelma Munir⁴, Rasheeda Bashir⁴

¹Department of Pharmacology and Toxicology; ²Department of Pharmacy, Hajvery University Lahore; ³Quality Operation Laboratory (QOL), University of Veterinary and Animal Sciences; ⁴Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan.

For correspondence:- Rasheeda Bashir Email: rashidasbs@yahoo.com Tel:+923074464628

Received: 26 August 2016 Accepted: 11 July 2017 Published: 31 August 2017

Citation: Najam K, Altaf I, Ashraf M, Rasheed MA, Saleem F, Munir N, et al. In vitro evaluation of mutagenicity and genotoxicity of sitagliptin alone and in combination with artificial sweeteners. Trop J Pharm Res 2017; 16(8):1841-1847 doi: 10.4314/tjpr.v16i8.13

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the in vitro genotoxicity and mutagenicity of sitagliptin alone and in combination with three commonly used artificial sweeteners (saccharin, aspartame and acesulfame-k).

Methods: The in vitro genotoxicity and mutagenicity of Sitagliptin alone and in combination with three popular artificial sweeteners (saccharin, aspartame and acesulfame-k) were evaluated by Comet and Ames assays, respectively.

Results: Sitagliptin demonstrated mutagenic potential only to TA 98 with S9 mix at a concentration of 3040 µg/plate. The mutagenicity of sitagliptin was enhanced when tested in combination with the artificial sweeteners. Furthermore, sitagliptin also caused pronounced DNA fragmentation at higher doses compared with negative control.

Conclusion: At higher doses, sitagliptin showed both mutagenicity and genotoxicity. Thus, long-term use of artificial sweeteners with sitagliptin may lead to increase in both mutagenicity and genotoxicity.

Keywords: Sitagliptin, Artificial sweeteners, Comet assay, DNA damage, Ames assay, Genotoxicity, Mutagenicity