Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Myricetin attenuates lung inflammation and provides protection against lipopolysaccharide-induced acute lung injury by inhibition of NF-KB pathway in rats

Mintao Mao, Mayu Huang

Emergency Department, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China;

For correspondence:- Mayu Huang Email: huangmayu167@hotmail.com Tel:+862152039999

Accepted: 4 October 2017 Published: 30 November 2017

Citation: Mao M, Huang M. Myricetin attenuates lung inflammation and provides protection against lipopolysaccharide-induced acute lung injury by inhibition of NF-KB pathway in rats. Trop J Pharm Res 2017; 16(11):2585-2593 doi: 10.4314/tjpr.v16i11.3

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate whether myricetin ameliorates lipopolysaccharide (LPS)-induced acute lung inflammation (ALI) in a rat model, and to elucidate the probable molecular mechanism of action involved.

Methods: Myricetin (10, 20 and 40 mg/kg) was administered to rats 30 min after intratracheally administering LPS (5 mg/kg). BALF protein concentration, lung wet-to-dry weight ratio, myeloperoxidase (MPO) activity, cytokine production and migration of inflammatory cells were evaluated.

Results: Myricetin significantly (p ≤ 0.05) attenuated lung inflammation as evident from the decreased wet-to-dry weight ratio of lungs, concentration of protein in the BALF, activity of MPO, cytokine production, and inflammatory cell migration. A decrease was also seen in TLR4, MyD88 and NF-κB expression. Additionally, an elevated antioxidant enzyme activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) was observed in all the treatment groups.

Conclusion: Myricetin provides protection against LPS-induced ALI. The underlying mechanisms of its anti-inflammatory action may include inhibition of NF-κB-mediated inflammatory responses.

Keywords: Acute lung injury, Anti-inflammatory, Myricetin, Inflammation, Cytokine, BALF, Flavonoid