Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro cytotoxicity of biosynthesized titanium dioxide nanoparticles in human prostate cancer cell lines

Feiping He^1,2, Weixing Yu², Xiaosong Fan², Baiye Jin¹ ,

¹Department of Urology, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang; ²Department of Urology, Shaoxing Shangyu Peopleâ€™s Hospital, Shaoxing 312300, Zhejiang, China.

For correspondence:- Baiye Jin Email: baiyejin@hotmail.com Tel:+8657187236666

Accepted: 23 November 2017 Published: 29 December 2017

Citation: He F, Yu W, Fan X, Jin B, In vitro cytotoxicity of biosynthesized titanium dioxide nanoparticles in human prostate cancer cell lines. Trop J Pharm Res 2017; 16(12):2793-2799 doi: 10.4314/tjpr.v16i12.2

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To establish a green method for production of titanium dioxide (TiO₂) nanoparticles (NPs) using Cinnamomum tamala (C. tamala) leaf extract, and examine the in vitro cytotoxicity of the product in a human prostate cancer (D145) cell line.

Methods: TiO₂ NPs were synthesized by mixing 20 mL of C. tamala leaf extract with 0.1 M titanium dioxide (Ti(OH)₂) (80 mL) in aqueous solution with stirring for 2 h at room temperature. The TiO₂ NPs were characterized using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), x-ray photoelectron spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), selected-area electron diffraction, and energy dispersive x-ray spectroscopy. The in vitro cytotoxicity against D145 cells was determined using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay.

Results: TEM and DLS analyses showed that the NPs were irregularly shaped, with an average particle size of 23 nm. The FT-IR spectrum of C. tamala leaf extract showed that the biomolecules were potentially involved in reduction processes. The negative zeta potential of -14 mV indicated that the NPs were stable and discrete while their crystalline nature was confirmed by XRD. Cytotoxicity analysis showed that the TiO₂ NPs exhibit a dose-dependent toxic effect on D145 cells.

Conclusion: A facile and less expensive approach for the production of TiO₂ NPs using C. tamala leaf extract has been developed. The TiO₂ NPs showed dose-dependent cytotoxicity towards D145 cells

Keywords: Anticancer activity, Cinnamomum tamala, Green synthesis, Prostate cancer, TiO2 nanoparticles