Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Qualitative and quantitative analysis of catechin and quercetin in flavonoids extracted from Rosa roxburghii Tratt

Ming-Hua Hao¹, Fan Zhang¹, Xing-Xia Liu², Fan Zhang¹, Li-Juan Wang¹, Sai-Juan Xu¹, Jin-Hua Zhang¹, Hong-Long Li³, Ping Xu¹

¹Department of Pharmacy; ²The Third Affiliated Hospital; ³Institute of Lung and Molecular Therapy, Xinxiang Medical University, Xinxiang, Henan, 453003, China.

For correspondence:- Ping Xu Email: 13273730271@163.com Tel:+8618240691447

Accepted: 6 October 2017 Published: 31 January 2018

Citation: Hao M, Zhang F, Liu X, Zhang F, Wang L, Xu S, et al. Qualitative and quantitative analysis of catechin and quercetin in flavonoids extracted from Rosa roxburghii Tratt. Trop J Pharm Res 2018; 17(1):71-76 doi: 10.4314/tjpr.v17i1.11

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To perform a qualitative and quantitative analysis of catechin and quercetin in flavonoids extracted from Rosa roxburghii Tratt.

Methods: Total flavonoids were determined using ultraviolet spectrophotometry (UV) at 500 nm. The optimal gradient program started with 15 % methanol and was kept within a period of 0 – 20 min, while 25 % methanol was kept within 20 – 33 min. Subsequently, the concentration of methanol was reduced to 15 % and was held for 10 min until the next injection. Mass spectrometry spray voltage was 4,000 V, ionization temperature 350 °C, atomizer pressure 35 psi, nitrogen flow rate 8 L/min, and mass scan range 200 – 800 m/z. The detection wavelength used for catechin and quercetin was 270 and 368 nm, respectively.

Results: Based on the UV results, Rosa roxburghii Tratt content was 73.85 %, which is in agreement with the national standard. Liquid chromatography-mass spectrometry (LC-MS) results indicate that Rosa roxburghii Tratt flavonoids contained quercetin, 34.26 %, with relative standard deviation (RSD) of 2.88 % and catechin content of 2.97 % with RSD of 1.49 %.

Conclusion: The proposed measurement method for determining the content of flavonoids in Rosa roxburghii Tratt has the advantage of simplicity, feasibility, good repeatability, and rapid and accurate analysis

Keywords: Rosa roxburghii Tratt, Flavonoids, Catechin, Quercetin