Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-inflammatory mechanism of Isodon japonicas (Burm) Hara on lipopolysaccharide-induced neuroinflammation

Hyun Kang

Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si, Chungnam, 31116, Republic of Korea;

For correspondence:- Email: hkang@dankook.ac.kr Tel:+82415503015

Published: 31 January 2018

Citation: Kang H. Anti-inflammatory mechanism of Isodon japonicas (Burm) Hara on lipopolysaccharide-induced neuroinflammation. Trop J Pharm Res 2018; 17(1):85-89 doi: 10.4314/tjpr.v17i1.13

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the anti-neuroinflammatory effects of Isodon japonicus (Burm.) Hara extract (IJE) on BV2 microglial cells. .

Methods: Cell viability was evaluated by MTT method. BV2 microglial cells were stimulated with lipopolyscarride (LPS, 1 µg/ml) and the effect of IJE on nitric oxide (NO) levels were measured using Griess assay. Immunoblot analysis was used to assess the effect of IJE on protein expression of inducible NO synthase (iNOS) expression. Tumor necrosis factor-alpha (TNF-α) cytokine production was evaluated by enzyme-linked immunosorbent assay (ELISA).

Results: Pretreatment of 100 mg/ml of IJE (p < 0.001) was inhibited nitric oxide (NO) by 1 ug/ml LPS-treated BV-2 cells. iNOS and TNF-α expression were attenuated by IJE concentration-dependently (p < 0.001 at 100 mg/ml). IJE scavenged 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependently with half-maximal inhibitory concentration (IC₅₀) value of 46.5 μg/ml.

Conclusion: Data from this study indicate that IJE attenuates neuroinflammatory responses. The strong anti-oxidant effect of IJE modulates expression of inflammatory molecules at the transcription level, and TNF-α at post-transcription level

Keywords: Isodon japonicas, Anti-oxidant, Neuroinflammation, BV-2 microglia, Nitric oxide