Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of electrolyzed reduced water on malondialdehyde levels and neutrophil cells in aggressive periodontitis

Markus B Rahardjo¹, Rini D Ridwan¹ , Delaneira AD Hikmah²

¹Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; ²Undergraduate Student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.

For correspondence:- Rini Ridwan Email: rini-d-r@fkg.unair.ac.id

Accepted: 21 January 2018 Published: 28 February 2018

Citation: Rahardjo MB, Ridwan RD, Hikmah DA. Effect of electrolyzed reduced water on malondialdehyde levels and neutrophil cells in aggressive periodontitis. Trop J Pharm Res 2018; 17(2):233-237 doi: 10.4314/tjpr.v17i2.6

© 2018 The authors.
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Abstract

Purpose: To evaluate the effects of electrolyzed reduced water (ERW) on malondialdehyde (MDA) levels and neutrophil cells in Wistar rats suffering from aggressive periodontitis.

Methods: Wistar rats (Rattus norvegicus) were infected with A. actinomycetemcomitans before being divided into a control group and a treatment group. The control group was treated orally with distilled water (pH 7.0), while the treatment group was administered with ERW (pH 9.8, 20 ml/day) for three successive days. Gingival tissue taken post-treatment was examined to determine the number of neutrophils and MDA level. Hematoxylin eosin staining was performed to identify neutrophil cells while MDA levels were determined by colorimetric assay.

Results: The number of neutrophils in the control group (30.04 ± 15.30) was higher than that of the treatment group (7.76 ± 2.52), while the MDA levels of the control and treatment groups were 4.58 ± 0.66 and 4.30 ± 0.81, respectively (p < 0.05).

Conclusion: ERW demonstrates the ability to reduce neutrophil cells without altering MDA levels in Wistar rats suffering from aggressive periodontitis. This is an indication that ERW was an effective material to manage aggressive periodontitis

Keywords: Periodontitis, Electrolyzed reduced water, Malondialdehyde, Neutrophil cells