Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Association of single nucleotide polymorphisms in miR-499 and miR-196a with susceptibility to breast cancer

Abdolhassan Doulah¹ , Ali Salehzadeh², Majid Mojarrad³

¹Department of Nursing, Faculty of Nursing and Midwifery, Ahvaz Branch, Islamic Azad University, Ahvaz; ²Department of Biology, Rasht Branch, Islamic Azad University, Rasht; ³Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

For correspondence:- Abdolhassan Doulah Email: h_doulah@yahoo.com Tel:+989163088656

Accepted: 2 January 2018 Published: 28 February 2018

Citation: Doulah A, Salehzadeh A, Mojarrad M. Association of single nucleotide polymorphisms in miR-499 and miR-196a with susceptibility to breast cancer. Trop J Pharm Res 2018; 17(2):319-323 doi: 10.4314/tjpr.v17i2.17

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the relationship between miR-499 rs3746444 and miR-196a rs11614913 polymorphisms, and susceptibility to breast cancer in an Iranian population.

Methods: This case-control study was performed on a population of 200 subjects comprising 100 breast cancer patients (case/observation group) and 100 healthy individuals (control group). Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was used to genotype these polymorphisms. P-values and odd ratios were determined, and p-values < 0.05 and odd ratios > 1 were considered statistically significant.

Results: There were no significant differences between observation and control groups with respect to rs11614913 T/C polymorphism. The rs11614913 T allele was not identified as a risk factor for susceptibility to breast cancer (OR = 0.86, 95 % CI = 0.85 - 1.3, p = 0.46). However, there were significant differences between observation and control groups with respect to rs3746444 T/C polymorphism. It was observed that cytosine-cytosine (CC) (OR = 4.5, 95 % CI = 1.3 - 15.4, p = 0.06), and cytosine-thymine (CT) (OR = 1.9, 95% CI = 1- 3.6, p = 0.04) genotypes had protective influence against susceptibility to breast cancer.

Conclusion: These results indicate that CC and CT genotypes are associated with reduced risk of breast cancer. In particular, the presence of C allele is significantly associated with a low risk of breast cancer. These findings may provide useful information for prevention and early diagnosis of breast cancer

Keywords: Breast cancer, Early diagnosis, miR-196a rs11614913, miR-499 rs3746444, Polymorphism, ARMS-PCR