Abdulwahab Noor Wali1,
Mohammed Farouk El Shal2 Farouk El Shal2,
Mayson H Alkhatib2 
,
Laila A Damiati3
For correspondence:- Laila Damiati Email:
Received: 17 April 2015 Accepted: 6 July 2015 Published: 30 August 2015
Citation: Wali AN, El Shal MF, Alkhatib MH, Damiati LA. Effect of three calmodulin antagonists on subpopulations of CD44/CD24 immunophenotypes in breast cancer cell lines. Trop J Pharm Res 2015; 14(8):1393-1398 doi: 10.4314/tjpr.v14i8.10
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To determine the effect of three calmodulin antagonists (A-7, W-7 and W-13) on the subpopulations of CD44/CD24 immunophenotypes in MDA-MB-231 and MDA-MB-468 breast cancer cell lines.
Methods: Flow cytometry analysis was used to determine the proportion of the various subpopulations of the immunophenotypes, viz, CD44+CD24-, CD44-CD24+ and CD44+CD24+, when MDA-MB-231 and MDA-MB-468 cells were subjected to calmodulin antagonists. The effect of W-13 on the invasion properties of MDA-MB-231 and MDA-MB-468 was investigated using Matrigel invasion assay.
Results: A-7, W-7 and W-13 caused alterations in the subpopulation of CD44+CD24- in MDA-MB-231 cells. The most potent antagonist was W-13 as it reduced the proportion of tumorigenic CD44+CD24- to 0.64 ± 0.05 at a concentration of 80 µM. In contrast, the subpopulation of MDA-MB-468 cells, which had a low fraction of CD44+CD24-, was not altered when administered with W-7 but showed variations when incubated with W-13. Specifically, when the concentration of W-13 increased from 20 – 100 µM, the proportion of CD44+CD24+ was reduced from 92.93 ± 3.2 to 60.96 ± 2.4. The effect of W-13 on the subpopulations of CD44+CD24- and CD44+CD24+ in MDA-MB-231 and MDA-MB-468, respectively, reduced the invasion properties of the cells.
Conclusion: The calmodulin antagonist, W-13, has a significant antitumor effect on MDA-MB-231 and MDA-MB-468 breast cancer cells.
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