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Original Research Article | OPEN ACCESS

Gentiana macrophylla Pall (Gentianaceae) extract exerts protective effects against osteoporosis in mice

Teng Gong1-3, Xue-Tao Su1 , Qun Xia1, Jing-Gui Wang1

1Department of Orthopedic Surgery, The Affiliated Hospital of Logistics College of Chinese PeopleR42;s Armed Police Force, No. 220 Chenglin Road, Dongli District, Tianjin City, 300162; 2Department of Spinal Surgery, Tianjin Hospital, Clinical Medical College of Tianjin Medical University, No. 406 Jiefang Nan road, Hexi District, Tianjin City, 300211; 3Post-doctoral Station, Tianjin Medical University, No. 22 Qixiang Tai Road, Tanjin City, 300070, China.

For correspondence:-  Xue-Tao Su   Email: skymberlysoul@yahoo.com   Tel:+862260578952

Accepted: 23 February 2018        Published: 31 March 2018

Citation: Gong T, Su X, Xia Q, Wang J. Gentiana macrophylla Pall (Gentianaceae) extract exerts protective effects against osteoporosis in mice. Trop J Pharm Res 2018; 17(3):429-434 doi: 10.4314/tjpr.v17i3.7

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the anti-osteoporotic effect of G. macrophylla extract in a mice model.
Methods: Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) for radical scavenging, nitro-blue tetrazolium (NBT) reducing, and ferric reducing antioxidant power (FRAP) assays using standard biochemical procedures. The expression of some bone-related genes was measured by quantitative real-time polymerase chain reaction (qTR-PCR). Phytochemical analysis was carried out by liquid chromatography-mass spectrometry (LC/MS).
Results: The extract exhibited strong antioxidant activity as evident from DPPH, NBT and FRAP data, with half-maximal inhibitory concentration (IC50) values of 275, 262 and 180 µg/ml, respectively. The antioxidant activity was concentration-dependent. The results showed that the extract restored bone characteristics by suppressing bone resorption through induction of expressions of several bone-associated genes. The results from phytochemical analyses showed the presence of loganic acid, swertiamarin, sweroside, gentiopicroside, gentesin and isogentesin.
Conclusion: These results indicate that the extract may be beneficial in the treatment of osteoporosis

Keywords: Gentiana macrophylla, Osteoporosis, Bone mass, Loganic acid, Swertiamarin, Sweroside, Gentiopicroside, Gentesin

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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