Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-inflammatory activity of Agaricus blazei Murill extract in the spleen of mice fed a high-fat diet

Dicky K Tontowiputro¹ , Djanggan Sargowo¹, Askandar Tjokroprawiro², Muhaimin Rifa'i³

¹Biomedical Department, Faculty of Medicine, Brawijaya University, Malang 65145; ²Surabaya Diabetes and Nutrition Center-Dr. Soetomo Public Hospital, Faculty of Medicine, Airlangga University, Surabaya 60286; ³Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, East Java, Indonesia.

For correspondence:- Dicky Tontowiputro Email: dickykurniawan.biomed@gmail.com Tel:+628560491

Accepted: 20 February 2018 Published: 31 March 2018

Citation: Tontowiputro DK, Sargowo D, Tjokroprawiro A, Rifa'i M. Anti-inflammatory activity of Agaricus blazei Murill extract in the spleen of mice fed a high-fat diet. Trop J Pharm Res 2018; 17(3):483-489 doi: 10.4314/tjpr.v17i3.14

© 2018 The authors.
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Abstract

Purpose: To examine the effects of A. blazei Murill on the production of proinflammatory cytokine TNF-α and regulatory cytokine IL-10 in the spleen of mice fed a high-fat diet.

Methods: The study was conducted on 25 BALB/c male mice divided into five groups consisting of five mice in each group and utilized three A. blazei Murill extract doses: 100, 200 and 400 mg/kg. The mice were fed a high-fat diet (HFD) and given A. blazei Murill extract for 12 weeks. The relative number of CD4⁺TNF-α⁺, CD11b/c⁺TNF-α⁺, and CD4⁺CD25⁺IL-10⁺ were measured using flow cytometry.

Results: Oral administration of A. blazei Murill extract (100 mg/kg) in mice fed a high-fat diet significantly decreased (p < 0.05) the level of TNF-α that produced by CD4⁺ T cells and macrophages (1.64 %), compared with control. The 200 mg/kg dose decreased the level of CD11b/c⁺TNF-α⁺ by 5.37 % (p < 0.05) compared to 100 mg/kg dose. The 400 mg/kg dose significantly enhanced regulatory cytokines IL-10 by 17.56 % (p < 0.05) in mice fed a high-fat diet.

Conclusion: This findings suggest that Agaricus blazei Murill can inhibits processes in HFD-induced mice by reducing TNF-α production and increasing the anti-inflammatory cytokine IL-10. These results provide new insight into the pharmacological actions of Agaricus blazei Murill as a medicinal food for potential therapy of atherosclerosis disease

Keywords: Anti-inflammatory agent, Agaricus blazei Murill, Proinflammatory cytokine, TNF-α, IL-10