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Original Research Article | OPEN ACCESS

Lentinan relieves hepatitis B surface antigen induced functional impairment of monocytes/macrophages

Pei-pei Fang1, Jie Li1, Shan-shan Huang1, Chen-wei Pan1, Guang-yao Zhou1, Lingxiang Jin1, Lu Zhuge1, Le Qin2

1Department of Infectious Disease; 2Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 325000, China.

For correspondence:-  Le Qin   Email: Qinle9105@163.com

Accepted: 26 March 2018        Published: 30 April 2018

Citation: Fang P, Li J, Huang S, Pan C, Zhou G, Jin L, et al. Lentinan relieves hepatitis B surface antigen induced functional impairment of monocytes/macrophages. Trop J Pharm Res 2018; 17(4):583-588 doi: 10.4314/tjpr.v17i4.3

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the efficacy of lentinan in relieving hepatitis B surface antigen (HBsAg)-induced functional impairment of monocytes/macrophages.
Methods: Monocytic cell line THP-1 was incubated with lentinan and HBsAg for 24 h and then stimulated with LPS (Lipopolysaccharide). The expression levels of interleukin-1β (IL-1β), IL-12 and tumour necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent (ELISA) assays and quantitative reverse transcriptase-PCR (q-PCR). Protein levels of IkB-α, phospho-ERK, and phospho-p38 were measured by western blotting.
Results: THP-1 cells treated with lentinan and HBsAg showed higher IL-1β, IL-12, and TNF-α levels than cells treated with HBsAg alone. The underlying mechanisms were associated with NF-kB and MAPK signal pathways. Decreased expression of IkB-α and phospho-ERK and increased expression of phospho-JNK and phospho-p38 were observed in cells treated with lentinan and HBsAg when compared with cells treated with HBsAg alone (p < 0.001). THP-1 cells incubated with 500 μg/mL lentinan secreted lower levels of cytokines than did control cells after LPS stimulation, suggesting an anti-inflammatory effect for lentinan.
Conclusion: Lentinan shows both pro- and anti-inflammatory functions and may be a promising candidate for hepatitis B virus (HBV) treatment.

Keywords: Hepatitis B surface antigen, Lentinan, Immuno-suppression, Pro-inflammatory, Anti-inflammatory

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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