Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Optimization of extraction of polyphenols from Sorghum Moench using response surface methodology, and determination of their antioxidant activities

Liu Liu¹, Long Chen¹, Arshad Mehmood Abbasi², Zichao Wang³, Dianxin Li⁴ , Yingbin Shen¹

¹Department of Food Science and Engineering, School of Science and Engineering, Jinan University, Guangzhou 510632, Guangdong, China; ²Department of Environmental Sciences, COMSATS Institute of information Technology, Abbottabad, Pakistan; ³College of Biological Engineering, Henan University of Technology, Zhengzhou 45000, Henan; ⁴Guangdong Vocational College of Science and Trade, Department of Biotechnology, Guangdong Province, Guangzhou 510000, China.

For correspondence:- Yingbin Shen Email: ldx2008403@126.com Tel:+8613660320534

Accepted: 22 March 2018 Published: 30 April 2018

Citation: Liu L, Chen L, Abbasi AM, Wang Z, Li D, Shen Y. Optimization of extraction of polyphenols from Sorghum Moench using response surface methodology, and determination of their antioxidant activities. Trop J Pharm Res 2018; 17(4):619-626 doi: 10.4314/tjpr.v17i4.8

© 2018 The authors.
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Abstract

Purpose: To employ response surface methodology (RSM) hinged on a central composite design (CCD) for the optimization of the extraction of polyphenols from Sorghum moench (Sorghum M).

Methods: The combined influence of independent variables were assessed with RSM. Total phenolic content (TPC) determination was carried out using Folin-Ciocalteu method. Derivative compounds of phenolic acid were assayed using high performance liquid (HPLC). Antioxidant potential was determined through 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging test.

Results: The optimized extraction conditions were: 60.37 % ethanol, temperature of 59.07 ^oC and 2.97 h of extraction duration, which resulted in the extraction of maximum amount of TPC, i.e., 313 mg GAE/100g dry weight. The interactions between temperature and ethanol concentration, and between extraction time and ethanol concentration had significant effects of TPC (p < 0.05). Under these conditions, there was a consistency between the projected and actual experimental levels of polyphenols. A positive correlation was found between TPC and DPPH radical scavenging activity (r=0.67, p <0.05). Furthermore, ferulic acid correlated positively with p-coumaric acid (r = 0.54, p <0.01).

Conclusion: These results underscore the usefulness of conditions for extraction in accurate quantification of antioxidants and phenolic compounds from Sorghum M, for possible application in large scale commercial extraction.

Keywords: Response surface methodology, Sorghum moench, Polyphenols, Antioxidants