Min Lv1,
Bo Li2,
Zhi-xuan Fu2,
Yu-ping Zhu2 
For correspondence:- Yu-ping Zhu Email: zhuyuping494@126.com Tel:+8657188128011
Accepted: 17 April 2018 Published: 28 May 2018
Citation: Lv M, Li B, Fu Z, Zhu Y. Gamellia sinensis O.Ktze extract shows anti-colorectal cancer activity via MAPK/ERK signaling pathway. Trop J Pharm Res 2018; 17(5):817-822 doi: 10.4314/tjpr.v17i5.10
© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the therapeutic effects of Gamellia sinensis O.Ktze extract (GSOE) on colorectal cancers, as well as the underlying mechanisms.
Methods: The effect of GSOE on colorectal cancer cells HCT-116 or Caco-2 growth was tested, and then the apoptosis and invasion was analyzed by MTT, flow cytometry and Transwell assay in vitro. Next, the mice received three doses (200, 400 or 800 mg/kg/day, gastric perfusion) of GSOE to evaluate its effects on tumor growth. Lung metastasis in mouse xenograft models which were inoculated with HCT-116 or Caco-2 cells were also investigated. The ex
Result: GSOE significantly inhibited colorectal cancer cell growth and induced apoptosis or cell cycle arrest at G1- and S-phases in HCT-116 cells and Caco-2 cells in a dose-dependent manner. Moreover, GSOE effectively retarded tumor cell migration and invasion through ERK/MAPK signaling pathway suppression.
Conclusion: These findings demonstrate that GSOE has an anti-tumor effect in colorectal cancer by inactivating ERK/MAPK signaling pathway.
Archives
News Updates
Fulltext in PDF